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Immunochemical Determination of Dioxins in Sediment Samples

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Abstract:Polychlorinated dibenzo-p-dioxins (PCDDs) and polychlorinated dibenzofurans (PCDFs) are considered highly toxic contaminants with the environmental monitoring of these compounds being of great importance. Immunoassay procedures such as the enzyme-linked immunosorbent assay (ELISA) may be useful as screening methods to satisfy the growing demand for rapid and low-cost analysis of these compounds. In this work, we describe the application of an immunoassay for the screening of PCDDs and PCDFs in sediments. Sample extraction and preparation methods were developed with the aim to establish the simplest, cost-effective and efficient removal of matrix interferences for the ELISA. The overall method is based on a hexane extraction; cleanup by a multilayered (H2SO4/NaOH) silica gel column followed by an activated carbon column; an organic solvent exchange with DMSO-0.01% Triton X-100, the final step being ELISA measurement. The less toxic 2,3,7-trichloro-8-methyldibenzo-p-dioxin (TMDD) was used as a surrogate standard for 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). The estimated theoretical method detection limit for the determination of PCDDs/PCDFs was about 20 pg TMDD equivalents per g of dry sediment. The accuracy (60-113%) and the precision (13-33% RSD) of the ELISA method were evaluated through the analysis of spiked sediment samples. Gas chromatography-high-resolution mass spectrometry (GC-HRMS) validation studies were performed using13 samples. The validation revealed that the method is suitable for Toxic Equivalent Quantity (TEQ) screening of dioxins in sediments with a method detection limit of about 100 pg TMDD g-1 sediment. The study indicated that the immunoassay screening method offers improvement in speed, sample throughput, as well as cost, in comparison to GC-HRMS analysis.

The United States Environmental Protection agency (EPA) through its Office of Research and Development funded and collaborated in the research described here under contract No.68-D 99-01 to Battelle. Although this work was reviewed by EPA and approved for publication, it may not necessarily reflect official Agency policy. Mention of trade names or commercial products does not constitute endorsement or recommendation for use.
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Citation:Nichkova, M., M. Chow, M. E. Koivunen, Kamita, S,G., S. J. Gee, J. C. Chuang, J. M. Van Emon, and B. D. Hammock. Immunochemical Determination of Dioxins in Sediment Samples. Presented at EPA EnviroExpo: Moving Toward Balance, Las Vegas, NV, December 9, 2004.
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Contact: Liz Hope - (919) 541-2785 or hope.elizabeth@epa.gov
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Division: Human Exposure & Atmospheric Sciences Division
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Branch: Exposure & Dose Research Branch
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Product Type: Abstrct/Oral
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Presented: 12/09/2004
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Related Entries:
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Bullet Item Field Analytical Methods: Advanced Field Monitoring Methods Development and Evaluation of New and Innovative Technologies That Support the Site Characterization and Monitoring Requirements of the Superfund Program.
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Last Updated on Monday, October 22, 2007
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