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Evaluation of Immunoassay Methods for Determination of 3,5,6-Trichloro-2-Pyridinol in Multiple Sample Media

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Abstract:Two enzyme-linked immunosorbent assay (ELISA) methods were evaluated for the determination of 3,5,6-trichloro-2-pyridinol (3,5,6-TCP) in multiple sample media (dust, soil, food, and urine). The dust and soil samples were analyzed by the RaPID (TM) commercial immunoassay testing kit based on antibody-coated magnetic particles. The food and urine samples were analyzed by a solid-phase 96-microwell plate immunoassay format. Methanol was used as the extraction solvent for the preparation of the dust and soil samples for analysis by both the ELISA and gas chromatography/mass spectrometry (GC/MS) procedures. Chlorobutane was used for the extraction of the urine samples for each method. The food samples were extracted with methanol for GC/MS and with acidic methanol for ELISA. The percent difference of the duplicate RaPID assays ranged from 0 to 43.4% for dust and from 0 to 47.9% for soil. The percent relative standard deviation of the 96-microwell plate triplicate assays was 15% or less for food and urine samples. Quantitative recoveries of 3,5,6-TCP were obtained for the spiked dust, soil, food and urine samples by the ELISAs ranging from 71 to 102%. Quantitative recoveries (>90%) of 3,5,6-TCP were also obtained for these samples by GC/MS. The overall method precision of these samples was within +/- 10% using the GC/MS procedure. The immunoassay and GC/MS data were highly correlated, with correlation coefficients of 0.98 for dust, 0.98 for soil, 0.93 for food and 0.98 for urine. Both ELISA methods can be used as quantitative monitoring tools for 3,5,6-TCP concentrations in dust, soil, food, and urine samples to reduce analytical costs and increase sample throughput.

Although this work was reviewed by EPA and approved for publication, it may not necessarily reflect official Agency policy. Mention of trade names or commercial products does not constitute endorsement or recommendation for use.
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Citation:Chuang, J. C., J. M. Van Emon, A. W. Reed, and N. Junod. Evaluation of Immunoassay Methods for Determination of 3,5,6-Trichloro-2-Pyridinol in Multiple Sample Media. Presented at EPA, Las Vegas EnviroExpo: Moving Towards Balance, Las Vegas, NV, December 9, 2004.
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Contact: Liz Hope - (919) 541-2785 or hope.elizabeth@epa.gov
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Division: Human Exposure & Atmospheric Sciences Division
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Branch: Exposure & Dose Research Branch
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Product Type: Abstrct/Oral
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Presented: 12/09/2004
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Related Entries:
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Bullet Item Human Exposure Measurements/Methods: Develop and Test Immunochemical and Other Screening Techniques for Use in Exposure Monitoring
spacer Relationship Reason:   A Project of the Product
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Last Updated on Monday, October 22, 2007
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