Skip common site navigation and headers
United States Environmental Protection Agency
Exposure Research
Begin Hierarchical Links EPA Home > Research & Development > Exposure Research > Publications/Presentations > End Hierarchical Links

 

Determination of a Bound Musk Xylene Metabolite in Carp Hemoglobin as a Biomarker of Exposure By Gas Chromatography Mass Spectrometry Using Selected Ion Monitoring

spacer
spacer
Abstract:Musk xylene (MX) is widely used as a fragrance ingredient in commercial toiletries. Identification and quantification of a bound 4-amino-MX (AMX) metabolite was carried out by gas chromatography-mass spectrometry (GC/MS), with selected ion monitoring (SIM). Detection of AMX occurred after the cysteine adducts in carp hemoglobin, derived from the nitroso metabolite, were released by alkaline hydrolysis. The released AMX metabolite was extracted into n-hexane. The extract was preconcentrated by evaporation, and analyzed by GC-SIM-MS. The concentration of AMX metabolite was found to range from 6.0 to 30.6 ng/g in the carp Hb, collected from the Las Vegas Wash and Lake Mead, Nevada areas. The presence of an AMX metabolite in the carp Hb was confirmed when similar mass spectral features and the same retention time of the AMX metabolite were obtained for both standard AMX and carp Hb extract solutions. In the non-hydrolyzed and reagent blank extracts, the AMX metabolite was not detected.
spacer
Citation:Mottaleb, M. A., W. C. Brumley, S. M. Pyle, and G. W. Sovocool. Determination of a Bound Musk Xylene Metabolite in Carp Hemoglobin as a Biomarker of Exposure By Gas Chromatography Mass Spectrometry Using Selected Ion Monitoring. JOURNAL OF ANALYTICAL TOXICOLOGY 28:581-586, (2004).
spacer
spacer
Contact: Chris Siebert - (702) 798-2234 or siebert.christopher@epa.gov
spacer
Division: Environmental Sciences Division
spacer
Branch: Environmental Chemistry Branch
spacer
Product Type: Journal
spacer
Published: 09/30/2004
spacer
Related Entries:
spacer
Bullet Item Addressing Emerging Issues in Water Quality Through Environmental Chemistry
spacer Relationship Reason:   A Project of the Product
spacer
spacer
spacer

 

ORD Home | Search EPA | Search NERL | Search EIMS | Contacts | Help

 
Begin Site Footer

EPA Home | Privacy and Security Notice | Contact Us

Last Updated on Monday, October 22, 2007
URL: http://cfpub.epa.gov