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ELISA Measurement for Stachylysin

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Abstract:The consumption of food commodities contaminated with the mold, Stachybotrys charatrum, has a long history of causing severe illness, and even death, in humans and animals. More recently, negative health effects have been linked to indoor inhalation exposures to S. chartarum. For these health effects to be documented, a mechanistic biomarker of exposure must be demonstrated. An enzyme linked- immunosorbent assay (ELISA) was developed for stachylysin, a proteinaceous hemolysin obtained from cultures of S. chartarum. The ELISA was highly selective to stachylysin and had an average limit of detection of 2 ppb depending upon the sample matrix. The conidia from several other molds showed a negligible reaction in the assay indicating the specificity of stachylysin to S. chartarum. The ELISA was used to analyze environmental samples from mold- contaminated buildings and biological samples obtained from individuals with known mold exposures. Stachylysin was quantified by the ELISA in spore, wallboard, mycelial and dust samples, and in the serum of adults exposed to S. chartarum in water-damaged environments. All control sera was negative for stachylysin. Thus, the analysis of stachylysin in serum may provide a useful measurement of human exposure to the mold. If stachylysin is a relevant biomarker, its determination in environmental samples may be useful for assessing and managing risk for indoor mold exposures.

Although this work was reviewed by EPA and approved for publication, it may not necessarily reflect official Agency policy. Mention of trade names or commercial products does not constitute endorsement or recommendation by EPA for use.
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Citation:Van Emon, J. M., and S. J. Vesper. ELISA Measurement for Stachylysin. Presented at American Society for Testing Materials, Boulder, CO, July 25-30, 2004.
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Contact: Liz Hope - (919) 541-2785 or hope.elizabeth@epa.gov
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Division: Human Exposure & Atmospheric Sciences Division
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Branch: Exposure & Dose Research Branch
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Product Type: Abstrct/Oral
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Presented: 07/29/2004
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Related Entries:
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Bullet Item Field Analytical Methods: Advanced Field Monitoring Methods Development and Evaluation of New and Innovative Technologies That Support the Site Characterization and Monitoring Requirements of the Superfund Program.
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Last Updated on Monday, October 22, 2007
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