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Mold Specific Quantitative Pcr for Rapid Identification and Enumeration

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Abstract:There is growing awareness that indoor molds/fungi may be connected to such conditions as asthma, allergies, hemorrhaging, chronic rhinosinusitis, memory loss, and a symptom complex called sick-building-syndrome. In addition, molds cause frequently fatal nosocomical infections. The US EPA has developed a mold specific quantitative PCR (MSQPCR) process that relies of the unique DNA signature of each mold species to do the identification and quantification. Assays are now available for more than 100 of the most common and relevant molds. These assays are standardized, specific, and sensitive (usually down to a single spore). The analysis can be completed in 2 to 3 hours. An automated platform can be used to perform these analyses. Examples of the use of the technology will be described in the presentation.
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Citation:Vesper, S. J. Mold Specific Quantitative Pcr for Rapid Identification and Enumeration. Presented at 7th Annual Army Force Health Protection (FHP) Confernce, Albuquerque, NM, August 6-12, 2004.
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Contact: Mary P. O'Bryant - (919)-541-4871 or obriant.mary@epa.gov
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Division: Microbiological & Chemical Exposure Assessment Division
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Branch: Microbial Exposure Research Branch
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Product Type: Sympos/Conf
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Presented: 08/06/2004
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Related Entries:
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Bullet Item Risk Assessment/Risk Management for Indoor Mold
spacer Relationship Reason:   A Project of the Product
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Last Updated on Monday, October 22, 2007
URL: http://cfpub.epa.gov