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A LC/MS Method for the Determination of Cyanobacteria Toxins in Water

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Abstract:The cyanobacteria toxins anatoxin-a, microcystin-LR, microcystin-RR, microcystin-YR, and nodularin were separated in less than 30 minutes on several 1 mm x 15 cm reverse phase liquid chromatography (LC) columns, and their electrospray mass spectra were measured with 50 ng or less injected amounts with a bench-top time-of-flight (TOF) mass spectrometer. New data from this work includes: (a) the impact of acetic acid concentrations in the methanol-water mobile phase on measured ion abundances; (b) the performance of the electrospray-TOF mass spectrometer as an LC detector; (c) the accuracy and precision of exact m/z measurements after LC separation with a routinely-used mass spectrometer resolving power of 5000; and, (d) recoveries of the five toxins from reagent water, river waters, and sewage treatment plant effluent samples extractged with C-18 silica particles enmeshed in thin Teflon (trademark) membrane filter disks. This technique has the otential of providing a relatively simple and reasonable-cost sample preparation and LC/MS method that provides the sensitivity, selectivity, reliability, and information content needed for drinking water occurrence and human exposure studies.
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Citation:Maizels, M., and W. L. Budde. A LC/MS Method for the Determination of Cyanobacteria Toxins in Water. Analytical Chemistry 76(5):1342-1351, (2004).
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Contact: Mary P. O'Bryant - (919)-541-4871 or obriant.mary@epa.gov
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Division: Microbiological & Chemical Exposure Assessment Division
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Branch: Immediate Office
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Product Type: Journal
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Published: 03/01/2004
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Related Entries:
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Bullet Item Toxins from Cyanobacteria in Water
spacer Relationship Reason:   A Project of the Product
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Last Updated on Monday, October 22, 2007
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