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Quantification of Enterovirus and Hepatitis a Viruses in Wells and Springs in East Tennessee Using Real-Time Reverse Transciption Pcr

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Abstract:This project involves development, validation testing and application of a fast, efficient method of quantitatively measuring occurrence and concentration of common human viral pathogens, enterovirus and hepatitis A virus, in ground water samples using real-time reverse transcription-polymerase chain reaction. Development of the real time RT-PCR assays for enterovirus and hepatitis A virus are complete and they are being verified by comparison with cell culture assays. The validation and application phase of the project includes monitoring viral occurrence and concentration in samples from six community water supply wells or springs in karst settings in east Tennessee. The wells/springs were chosen on the basis of prior monitoring of fecal indicator bacteria and geochemical parameters (temperature, turbitity, DO, etc.), as well as the presence or absence of likely input sources of human sewage. The wells/springs used for sampling include two sites that have a relatively high likelihood of containing human pathogens, two that have a very low likelihood, and two intermediate sites. Sampling for viruses is scheduled to begin in January 2004 and these are expected to be the first measurements of viral occurrence in groundwater in the state of Tennessee.
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Citation:Baldwin, T., A. C. Layton, L. D. Mckay, S. Jones, V. Garrett, G. Johnson, and G. S. Fout. Quantification of Enterovirus and Hepatitis a Viruses in Wells and Springs in East Tennessee Using Real-Time Reverse Transciption Pcr. Presented at 4th International Groundwater Quality Conference, Ontario, Canada, July 19-22, 2004.
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Contact: Mary P. O'Bryant - (919)-541-4871 or obriant.mary@epa.gov
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Division: Microbiological & Chemical Exposure Assessment Division
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Branch: Biohazard Assessment Research Branch
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Product Type: Abstrct/Oral
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Presented: 07/19/2004
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Related Entries:
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Bullet Item Measure Occurrence and Exposure to Waterborne Human Viruses
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Last Updated on Monday, October 22, 2007
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