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Real-Time Pcr Method to Detect Enterococcus Faecalis in Water

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Abstract:A 16S rDNA real-time PCR method was developed to detect Enterococcus faecalis in water samples. The dynamic range for cell detection spanned five logs and the detection limit was determined to be 6 cfu/reaction. The assay was capable of detecting E. faecalis cells added to biofilms from a simulator of a water distribution system and in freshwater samples. Nucleic acid extraction was not required, permitting the detection of E. faecalis cells in less than 3 h.
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Citation:Santo Domingo, J., S. D. Siefring, and R. A. Haugland. Real-Time Pcr Method to Detect Enterococcus Faecalis in Water. BIOTECHNOLOGY LETTERS 25(3):261-265, (2003).
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Contact: Mary P. O'Bryant - (919)-541-4871 or obriant.mary@epa.gov
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Division: Microbiological & Chemical Exposure Assessment Division
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Branch: Microbial Exposure Research Branch
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Product Type: Journal
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Published: 02/01/2003
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Related Entries:
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Bullet Item Rapid, Pcr-Based Methods for Measuring the Quality of Bathing Beach Waters
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Last Updated on Monday, October 22, 2007
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