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Determining Eosin as a Groundwater Migration Tracer By Capillary Electrophoresis/Laser-Induced Fluorescence Using a Multiwavelength Laser

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Abstract:Groundwater migration remains an important contributor in determining the distribution and fate of environmental pollutants originating from various waste sites or in understanding fate and transport .[ 1- 3] .Groundwater tracers are often used to determine the flow of groundwater. The tracers can be fluorescent dyes, in which case the use of synchronous scanning spectrofluorimetry is the technique of choice to determine the tracers in water or other samples. The U.S. EPA National Exposure Research Laboratory (Las Vegas) is interested in developing and applying 8 analytical tools that can strengthen the regulatory application of analysis. Among those tools under investigation is capillary electrophoresis/laser-induced fluorescence detection (CE/LIF), which is particularly well-suited to the analysisofwater-soluble and fluorogenic compounds. Fluorescent dyes are a convenient choice for tracers because of the ease of sensitive detection. Spectrofluorimetry , high performance liquid chromatography (HPLC)/UV or fluorescence detection, and capillary LC/fluorescence detection have also been used [2-6]. For HPLC or capillary LC, the retention of dye analytes is enhanced using ion-pairing techniques. Logically, applications of capillary electrophoresis/laser-induced fluorescence (CE/LIF) detection should be ideal for the determination of anionic ( or cationic) dyes. In free-zone CE, the dyes are separated very simply on the basis of their mobilities in aqueous buffers. Among several reports on CE/LIF detection techniques are two early papers based on high-sensitivity detection of fluorescent dyes [7,8] and more recent work applied directly to groundwater migration studies [9-12] .General reviews of environmental applications of CE and sample handling were published [13-14], and more recently updated reviews appear regularly [15] Additional environmental
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Citation:Brumley, W. C., and J. W. Farley. Determining Eosin as a Groundwater Migration Tracer By Capillary Electrophoresis/Laser-Induced Fluorescence Using a Multiwavelength Laser. ELECTROPHORESIS 24(14):2335-2339, (2003).
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Contact: Chris Siebert - (702) 798-2234 or siebert.christopher@epa.gov
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Division: Environmental Sciences Division
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Branch: Environmental Chemistry Branch
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Product Type: Journal
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Published: 07/30/2003
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Related Entries:
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Bullet Item Advances in Sampling, Separation, Detection and Identification That Address Unmet Needs of Oswer and the Regions
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