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Closed-Loop Stripping Analysis (Clsa) of Synthetic Musk Compounds from Fish Tissues With Measurement By Oc/MS/Sim

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Abstract:Synthetic musk compounds are used as inexpensive fragrance materials for the production
of perfumes and as additives to soap, detergent, and shampoo. They have been found in surface water, fish tissues, and human breast milk. The ubiquity of this class of compounds in the environment is attributable to high use and release into the environment. Current techniques for separating these compounds from fish tissues require tedious sample clean-up procedures. To obtain fat-free extracts, gel permeation chromatography (GPC), column chromatography using alumina, and silica gel, and thin layer chromatography (TLC clean-up procedures are frequently employed. Despite the considerable effort and resources devoted to these processes, a fraction of the lipids and lipid-like compounds frequently remains in the extracts. These low-level lipids foul injection liners, contaminate columns, and yield elevated baselines during gas chromatographic analysis of synthetic musk compounds. In this study, a simple method for the determination of synthetic musk compounds in fish tissues has been developed. Closed-loop stripping of saponified fish tissues in a I -L Wheaton purge- and-trap vessel, is used to strip compounds with high vapor pressures such as synthetic musks from the matrix onto a solid sorbent (Abselut Nexus). This technique is useful for screening biological
tissues that contain lipids for musk compounds. Analytes are desorbed from the sorbent trap
sequentially with polar and nonpolar solvents, concentrated, and directly analyzed by high resolution gas chromatography coupled to a mass spectrometer operating in the selected multiple-ion monitoring mode. In this paper, we analyzed two homogenized samples of whole fish tissues with spiked synthetic musk compounds using either closed-loop stripping analysis (CLSA) or accelerated solvent extraction (ASE). The recoveries for the two techniques are presented.
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Citation:Osemwengie, L. I., and S. Steinberg. Closed-Loop Stripping Analysis (Clsa) of Synthetic Musk Compounds from Fish Tissues With Measurement By Oc/MS/Sim. JOURNAL OF CHROMATOGRAPHY A 993(1-2):1-15, (2003).
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Contact: Chris Siebert - (702) 798-2234 or siebert.christopher@epa.gov
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Division: Environmental Sciences Division
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Branch: Environmental Chemistry Branch
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Product Type: Journal
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Published: 04/30/2003
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Related Entries:
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Bullet Item Addressing Emerging Issues in Water Quality Through Environmental Chemistry
spacer Relationship Reason:   A Project of the Product
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