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A Multiplex Reverse Transciption-Pcr Method for Detection of Human Enteric Viruses in Groundwater

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Abstract:Untreated groundwater is responsible for about half of the waterborne disease outbreaks in the United States. Human enteric viruses are thought to be leading etiological agents of many of these outbreaks, but there is relatively little information on the types and levels of viruses found in groundwater. To address this problem, monthly samples from 29 groundwater sites were analyzed for one year for enteroviruses, hepatitis A virus (HAV), Norwalk virus, reovirouses and rotaviruses by multiplex reverse transcription-PCR (RT-PCR). A procedure was developed to remove environmental inhibitors of RT-PCR from groundwater samples. The procedure allowed an average of 71 liters of the original groundwater to be asssayed per RT-PCR reaction with an average virus recovery rate of 74%, based upon seeded samples. Human enteric viruses were detected in 16% of the groundwater samples analyzed with reoviruses being the most frequently detected virus group.
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Citation:Fout, G. S., B. C. Martinson, M. N. Moyer, D. R. Dahling, and L. J. Wymer. A Multiplex Reverse Transciption-Pcr Method for Detection of Human Enteric Viruses in Groundwater. APPLIED AND ENVIRONMENTAL MICROBIOLOGY 69(6):3158-3164, (2003).
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Contact: Mary P. O'Bryant - (919)-541-4871 or obriant.mary@epa.gov
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Division: Microbiological & Chemical Exposure Assessment Division
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Branch: Biohazard Assessment Research Branch
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Product Type: Journal
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Published: 06/01/2003
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Related Entries:
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Bullet Item Measure Occurrence and Exposure to Waterborne Human Viruses
spacer Relationship Reason:   A Project of the Product
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Last Updated on Monday, October 22, 2007
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