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Low Pressure Ultraveiolet Studies for Inactivation of Giardia Muris Cysts

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Abstract:Cysts of Giardia muris were inactivated using a low pressure ultravolet (UV) light source. Cyst viability was detemined by both in vitro excystation and animal infectivity. Cyst doeses were counted using a flow cytometer for the animal infectivity experiments. Using in vitro excystation as the viability indicator, fluences as high as approximately 200 mJ/cm(2) did not prevent some cysts from excysting, thus verifying earlier work. Using animal infectivity, UV fluences of 1.4, 1.9 and 2.3 mJ/cm(2) yielded log(10) reductions ranging from 0.3 to greater than or equal to 4.4. Results indicate in vitro excystation is not a reliable indicator of G. muris cyst viability after UV disinfection.
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Citation:Hayes, S. L., E. W. Rice, M. W. Ware, and F. W. Schaefer III. Low Pressure Ultraveiolet Studies for Inactivation of Giardia Muris Cysts. JOURNAL OF APPLIED MICROBIOLOGY 94(1):54-59, (2003).
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Contact: Mary P. O'Bryant - (919)-541-4871 or obriant.mary@epa.gov
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Division: Microbiological & Chemical Exposure Assessment Division
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Branch: Biohazard Assessment Research Branch
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Product Type: Journal
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Published: 01/01/2003
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Last Updated on Monday, October 22, 2007
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