Skip common site navigation and headers
United States Environmental Protection Agency
Exposure Research
Begin Hierarchical Links EPA Home > Research & Development > Exposure Research > Publications/Presentations > End Hierarchical Links

 

Human Blood and Environmental Media Screening Method for Pesticides and Polychlorinated Biphenyl Compounds Using Liquid Extraction and Gas Chromatography-Mass Spectrometry Analysis

spacer
spacer
Abstract: Screening assessment methods have been developed for semi- and non-volatile persistent organic pollutants (POPs) for human blood and solid environmental media. The specific methodology is developed for measuring the presence of "native" compounds, specifically, a variety of organochlorine pesticides (OCPs), organophosphate pesticides (OPPs), and for polychlorinated biphenyls (PCBs). The method is demonstrated on anonymous Red Cross blood samples as well as two potential environmental sources, tracked in soil and dog hair. This work is based on previously developed methods for semi-volatile hydrocarbon exposure from fuels usage and similarly employs liquid solvent extraction, evaporative volume reduction, and subsequent specialized gas chromatography - mass spectrometry analysis (GC-MS). Standard curves, estimates of recovery efficiency, and specific GC-MS SIM quantization methods were developed for common pesticides including diazinon, aldrin, chlorpyrifos, malathion, dieldrin, DDT, permethrin, cyhalothrin, and cypermethrin, and for seven selected PCBs. Trace levels of certain PCBs and pesticides such as permethrin, dieldrin, malathion, lindane, diazinon, and chlorpyrifos were tentatively identified in anonymous blood samples as well as in two potential environmental sources, tracked in soil and dog hair. The method provides a simple screening procedure for various media and a variety of common organic pollutants without extensive sample preparation. It is meant to complement and augment data from more specific or complex methodology, to provide initial broad spectrum guidance for designing targeted experiments, and to provide confirmatory evidence for the ususal metabolic biomarker measurements made to assess human exposure.

This work was funded by the National Exposure Research Laboratory, Office of Research and Development, U.S. Environmental Protection Agency, through Contract 68-D5-0049 with ManTech Environmental Technology, Inc. Mention of trade names or commercial products does not constitute endorsement or recommendation for use.
spacer
Citation:Liu, S., and J. D. Pleil. Human Blood and Environmental Media Screening Method for Pesticides and Polychlorinated Biphenyl Compounds Using Liquid Extraction and Gas Chromatography-Mass Spectrometry Analysis. JOURNAL OF CHROMATOGRAPHY: B BIOMEDICAL SCIENCES AND APPLICATIONS 769(1):155-167, (2002).
spacer
spacer
Contact: Liz Hope - (919) 541-2785 or hope.elizabeth@epa.gov
spacer
Division: Human Exposure & Atmospheric Sciences Division
spacer
Branch: Exposure Methods & Monitoring Branch
spacer
Product Type: Journal
spacer
Published: 03/15/2002
spacer
Related Entries:
spacer
Bullet Item Xenobiotic Methods Development for Human Exposure Assessment Research
spacer Relationship Reason:   A Project of the Product
spacer
spacer
spacer

 

ORD Home | Search EPA | Search NERL | Search EIMS | Contacts | Help

 
Begin Site Footer

EPA Home | Privacy and Security Notice | Contact Us

Last Updated on Monday, October 22, 2007
URL: http://cfpub.epa.gov