Skip common site navigation and headers
United States Environmental Protection Agency
Exposure Research
Begin Hierarchical Links EPA Home > Research & Development > Exposure Research > Publications/Presentations > End Hierarchical Links

 

Immunocytochemical Localization of Stachylysin in Stachybotrys Chartarum Spores and Spore-Impacted Mouse and Rat Lung Tissues

spacer
spacer
Abstract:Stachylysin is a proteinaceous hemolytic agent that is producted by S. chartarum. Stachylysin was found, using immunohistochemistical and immunocytochemical methods, to be localized in S. chartarum spores/mycelia primarily in the inner wall suggesting that it is constitutively produced. Spores instilled in mouse or rat lung tissues resulted in granuloma formations which showed the highest stachylysin concentration in the inner wall of the spore, with less at distance indicating that it has diffused out from the spore. The in vitro high stachylysin producing strain (58-06) was also highest in vivo, based on immunohistochemistical staining. More stachylysin was observed in the lung tissue at 72 h than at 24 h indicating that production /release is a relatively slow process. The localization of stachylysin in macrophage phagolysosomes suggests that these cells may be involved with hemolysin inactivation. This would be consistent with what is know about asp-hemolysin produced by Aspergillus fumigatus.
spacer
Citation:Gregory, L., T. G. Rand, D. Dearborn, I. Yike, and S. J. Vesper. Immunocytochemical Localization of Stachylysin in Stachybotrys Chartarum Spores and Spore-Impacted Mouse and Rat Lung Tissues. MYCOPATHOLOGIA 156(2):109-117, (2003).
spacer
spacer
Contact: Mary P. O'Bryant - (919)-541-4871 or obriant.mary@epa.gov
spacer
Division: Microbiological & Chemical Exposure Assessment Division
spacer
Branch: Microbial Exposure Research Branch
spacer
Product Type: Journal
spacer
Published: 03/01/2003
spacer
Related Entries:
spacer
Bullet Item Risk Assessment/Risk Management for Indoor Mold
spacer Relationship Reason:   A Project of the Product
spacer
spacer
spacer

 

ORD Home | Search EPA | Search NERL | Search EIMS | Contacts | Help

 
Begin Site Footer

EPA Home | Privacy and Security Notice | Contact Us

Last Updated on Monday, October 22, 2007
URL: http://cfpub.epa.gov