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Capillary Electrophoresis Immunoassay for 2,4-Dichlorophenoxyacetic Acid

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Abstract: A capillary electrophoresis (CE) immunoassay format for 2,4-dichlorophenoxyacetic acid (2,4-D) is demonstrated. A fluorescent labeled 2,4-D analog competes with the analyte of interest for a finite number of binding sites provided by anti-2,4-D monoclonal antibodies. CE then provides a means of separating and measuring both the free and antibody-bound fluorescent tracer using laser-induced fluorescence detection. For this assay format, the amount of free tracer is a sensitive indicator for the concentration of analyte present in the sample. A sequential injection format allows the rapid analysis of a small number of samples. The dynamic concentration range for 2,4-D in either buffer or river water is 5 ppb to 1000 ppb.

The U.S. Environmental Protection Agency (EPA), through its Office of Research and Development (ORD), funded the work involved in preparing this article. It has been subject to the Agency's peer review and has been approved for publication. Mention of trade names or commercial products does not constitute endorsement or recommendation by the U.S. EPA.
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Citation:Rogers, K. R., A. B. Apostol, and W. C. Brumley. Capillary Electrophoresis Immunoassay for 2,4-Dichlorophenoxyacetic Acid. ANALYTICAL LETTERS 33(3):443-453, (2000).
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Contact: Liz Hope - (919) 541-2785 or hope.elizabeth@epa.gov
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Division: Human Exposure & Atmospheric Sciences Division
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Branch: Human Expsoure Research Branch
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Product Type: Journal
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Published: 01/31/2000
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Last Updated on Monday, October 22, 2007
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