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Improved Selective Electrocatalytic Oxidation of Phenols By Tyrosinase-Based Carbon Paste Electrode Biosensor

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Abstract: Tyrosinase-based carbon paste electrodes are evaluated with respect to the viscosity and polarity of the binder liquids. The electrodes constructed using a lower viscosity mineral oil yielded a greater response to phenol and catechol than those using a higher viscosity oil of similar chemical composition. Despite its relatively high viscosity, the use of silicon oil Si PS 086 as carbon paste binder resulted in a 2-fold increase over Si AR 200 and a 4?6 fold increase (over mineral oils) in responses to phenol, p-chlorophenol, and p-cresol. Also reported is the enhanced enzyme electrode response to phenol and catechol resulting from pre-oxidation of these substrates using a plain carbon paste electrode in a series (in-line) dual electrode configuration.

The US Environmental Protection Agency (EPA), through its Office of Research and Development (ORD), funded this research. It has been subject to the EPA's peer and administrative review and has been approved for publication. Mention of trade names or commercial products does not constitute endorsement or recommendation by EPA for use.
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Citation:Rogers, K. R., J. Y. Becker, and J. Cembrano. Improved Selective Electrocatalytic Oxidation of Phenols By Tyrosinase-Based Carbon Paste Electrode Biosensor. ELECTROCHIMICA ACTA 45(25-26):4373-4379, (2000).
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Contact: Liz Hope - (919) 541-2785 or hope.elizabeth@epa.gov
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Division: Human Exposure & Atmospheric Sciences Division
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Branch: Human Expsoure Research Branch
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Product Type: Journal
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Published: 11/16/2000
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Related Entries:
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Bullet Item Biosensors Research for Development of Innovative Monitoring Techniques That Support Exposure Assessment Related to the Superfund Program
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Last Updated on Monday, October 22, 2007
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