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Detection of Cryptosporidium Oocysts in Stream Water Samples Using U.S. Environmental Protection Agency Method 1622

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Abstract:To improve surveillance for cryptosporidium oocysts in water the USEPA developed Method 1622 which consists of filtration, concentration, immunomagnetic separation, fluorescent antibody and DAPI counter staining, and microscopic evaluation. Two filters were compared for analysis of 11 stream water samples collected throughout the U.S. Replicate 10--L stream water samples, unspiked and spiked with 100-250 oocysts, were tested to evaluate matrix effects. Oocyst recoveries from the stream water samples averaged 22% and 12% with a membrane disk and a capsule filter, respectively. These results demonstrate that cryptosporidium oocysts can be recovered from stream waters using Method 1622 but recoveries are lower than from reagent-grade water. Concentrations of indicator bacteria also were evaluated in these steam water samples. Because few samples were oocyst-positive, relationships between detections of oocysts and concentrations of indicator organisms could not be determined.
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Citation:Simmons, O. D., C. D. Heaney, D. S. Francy, R. Nally, F. W. Schaefer, and M. D. Sobsey. Detection of Cryptosporidium Oocysts in Stream Water Samples Using U.S. Environmental Protection Agency Method 1622. JOURNAL OF THE AMERICAN WATER WORKS ASSOCIATION 67(3):1123-1127, (2001).
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Contact: Mary P. O'Bryant - (919)-541-4871 or obriant.mary@epa.gov
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Division: Microbiological & Chemical Exposure Assessment Division
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Branch: Biohazard Assessment Research Branch
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Product Type: Journal
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Published: 01/01/2001
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Related Entries:
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Bullet Item Detecting Ccl-Related, Emerging and Regulated Waterborne Human Protozoa for Exposure Assessment
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Last Updated on Monday, October 22, 2007
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