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Method to Classify Environmental Samples Based on Mold Analyses By Qpcr

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Abstract:A total of 82 quantitative PCR (QPCR) assays were used to identify and quantify different indoor molds in dust samples from the homes of six infants suffering from pulmonary hemorrhage and 26 reference homes in Cleveland, Ohio. No significant difference was seen in the total cell quantities (presumed to be primarily spores and conidia) of these molds in the reference home (RH) and pulmonary hemorrhage home (PHH) samples. The assay groups were categorized based on whether the ratio of measured organisms in the PHH and RH samples were <2 or > 2. Twenty-two groups were identified in the >2 category. The combined cell quantities of these groups were significantly higher in the PHH samples than in the RH samples (p=0.001) and comprised 4% of all detected organisms in the PHH samples.
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Citation:Haugland, R. A., T. Meklin, M. Varma, L. J. Wymer, D. G. Dearborn, I. Yike, and S. J. Vesper. Method to Classify Environmental Samples Based on Mold Analyses By Qpcr. Presented at Bioaerosols Meeting, Saratoga, NY, September 10-12, 2003.
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Contact: Mary P. O'Bryant - (919)-541-4871 or obriant.mary@epa.gov
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Division: Microbiological & Chemical Exposure Assessment Division
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Branch: Microbial Exposure Research Branch
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Product Type: Sympos/Conf
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Presented: 09/10/2003
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Related Entries:
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Bullet Item Risk Assessment/Risk Management for Indoor Mold
spacer Relationship Reason:   A Project of the Product
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Last Updated on Monday, October 22, 2007
URL: http://cfpub.epa.gov