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Wet Demonstration Workshop on the Identification of Cryptosporidium, Microsporidia, and Giardia in Drinking Water

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Abstract:This method is for determination of the identity and concentration of Cryptosporidium (CAS Registry number 137259-50-8) and Giardia (CAS Registry number 137259-49-5) in untreated surface water and in other waters by filtration, immunomagnetic separation (IMS), and immunofluorescence assay (FA) microscopy. Cryptosporidium and Giardia may be confirmed using 4',6-diamidino-2-phenylindole (DAPI) staining and differential interference contract (D.I.C.) microscopy. This method is designed to meet the survey and monitoring requirements of the U.S. Environmental Protection Agency (EPA). It is based on laboratory testing of recommendations by a panel of experts convened by EPA. The panel was charged with recommending an improved protocol for recovery and detection of protozoa that could be tested and implemented with minimal additional research. This method will not identify the species Cryptosporidium or Giardia or the host species of origin, nor can it determine the viability or infectivity of detected oocysts and cysts. This method is for use only by persons experienced in the determination of Cryptosporidum and Giardia by filtration, IMS, and FA. experienced persons are defined in Section 22.0 at the end of this method as the principal analyst/supervisor, analyst, and technician. Laboratories unfamiliar with analyses of environmental samples by the techniques in this method should gain experience using water filtration techniques, IMS, fluorescent antibody staining with monoclonal antibodies, and microscopic examination of biological particulates using bright-field and D.I.C. microscopy.
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Citation:Schaefer III, F. W., and H. A. Lindquist. Wet Demonstration Workshop on the Identification of Cryptosporidium, Microsporidia, and Giardia in Drinking Water. Presented at VII International Workshop on Opportunistic Protists, Cincinnati, OH, June 13, 2001.
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Contact: Mary P. O'Bryant - (919)-541-4871 or obriant.mary@epa.gov
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Division: Microbiological & Chemical Exposure Assessment Division
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Branch: Biohazard Assessment Research Branch
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Product Type: Sympos/Conf
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Presented: 06/13/2001
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Last Updated on Monday, October 22, 2007
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