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Development of a Molecular Method to Identify Astrovirus in Water.

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Abstract:Astrovirus is a common cause of gastroenteritis that has been determined to be responsible for several outbreaks. Since astrovirus can be waterborne, there is interest in testing environmental water for astrovirus and we have developed a sensitive RT-PCR assay that is designed to detect all known astrovirus. When tested, this assay was able to detect strains from all eight serotypes. In addition, an internal control was developed so that it will be possible to determine if the sample being tested contains PCR inhibitors. Most probable number analysis determined that when amplified with the developed assay, a single DNA molecule of the internal control could be detected if inhibitors were not present. The assay was successfully adapted to real-time PCR and this method was then used for integrated cell culture RT-PCR detection of infectious virus. The methods were successfully used to detect astrovirus present in clinical samples and spiked water samples.
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Citation:Grimm, A. C., J. L. Cashdollar, F. P. Williams, and G. S. Fout. Development of a Molecular Method to Identify Astrovirus in Water. Presented at Science and Mission Club, Cincinnati, OH, June 4, 2003.
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Contact: Mary P. O'Bryant - (919)-541-4871 or obriant.mary@epa.gov
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Division: Microbiological & Chemical Exposure Assessment Division
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Branch: Biohazard Assessment Research Branch
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Product Type: Abstrct/Oral
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Presented: 06/04/2003
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Related Entries:
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Bullet Item Detecting Ccl-Related, Emerging Waterborne Human Viruses and Viral Indicators for Exposure Assessment
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Last Updated on Monday, October 22, 2007
URL: http://cfpub.epa.gov