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Investigatoin of Cyanobacteria Toxins in Water

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Abstract:Introduction:

Approximately 80 alkaloid and cyclic peptide toxins produced by various freshwater and marine cyanobacteria (blue-green algae) have been identified and their structures determined. The U. S. Environmental Protection Agency has identified two neurotoxin alkaloids and four cyclic peptide toxins that have the potential for serious contamination of sources of drinking water for humans and animals. The purpose of this research is to develop a convenient, rapid, but definitive analytical method for these toxins in water.

Methods and Instrumentation:

Four of the six toxins of interest, and three of lessor interest, were available for this work. Anatoxin-a, microcystins RR, LR, and YR, and nodularin were added to laboratory reagent water and to samples of natural waters at environmentally significant concentrations. The analytes were separated from the water by liquid-solid extraction using C-18 silica impregnated filter disks, eluted from the disks with methanol, and the eluate was analyzed using microbore (1 mm ID) LC combined with electrospray and time-of-flight mass spectrometry.

Preliminary Data:

The analytes anatoxin-a, microcystins RR, LR, and YR, and the cyclic peptide nodularin in methanol are separated in about 25 minutes. With about 50 ng of each injected, the LC peaks in the TIC have very good S/N. Microcystin RR gives mainly the (M+2H]2+ ion and the other analyes give the corresponding singly charged ions. In source CID gives a characteristic m/z 135 ion for the microcystins and nodularin, but anatoxin-a does not have the necessary structural feature to give this ion. Mean recoveries from laboratory water for the microcystins and nodularin were in the 85-98% range with RSDs in the 5- 17% range. The mean recovery of anatoxin-a was just 68% (RSD 11%). A suitable internal standard has not been identified and external standardization may account for some of the recoveries and analytical presicion.

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Citation:Budde, W. L., and M. Maizels. Investigatoin of Cyanobacteria Toxins in Water. Presented at Annual Conference of the American Society for Mass Spectrometry, Montreal, CA, June 8-13, 2003.
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Contact: Mary P. O'Bryant - (919)-541-4871 or obriant.mary@epa.gov
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Division: Microbiological & Chemical Exposure Assessment Division
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Branch: Immediate Office
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Product Type: Abstrct/Oral
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Presented: 06/08/2003
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Related Entries:
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Bullet Item Toxins from Cyanobacteria in Water
spacer Relationship Reason:   A Project of the Product
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Last Updated on Monday, October 22, 2007
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