Skip common site navigation and headers
United States Environmental Protection Agency
Exposure Research
Begin Hierarchical Links EPA Home > Research & Development > Exposure Research > Publications/Presentations > End Hierarchical Links

 

Use of Long Acting Steroid Methylprednisolone Acetate for the Prolongation of Cryptosporidium Sp. in Mice

spacer
spacer
Abstract:Current protocols for Cryptosporidium sp. propagation in mice vary according to the strain and age of mouse as well as the species of Cryptosporidium. Cryptosporidium muris is a natural parasite of mice, but only neonatal mice are susceptible to C. parvum infection. Published C. parvum protocols for adult mice usually involve the use of the water soluble form of dexamethazone, administered daily or every other day, either ad libitum in the drinking water or by intra peritoneal injection. Uniformity of doses a problem with any water bottle delivery system. Also, excessive animal handling and labor costs are significant with daily injections.

In an attempt to decrease handling problems and dosing variations a single injection method was developed. A single injection of Methylprednisolone Acetate (DepoMedrol - Pharmacia-UpJohn) is sufficient to allow a Cryptosporidium parvum infection in adult CF-1 mice. The effective dose appears to increase with the age and weight of the mouse. In mice naturally infected with Cryptosporidium muris a single dose prior to infection will prolong the shedding of oocysts. A single dose after the prepatient period will radically incrase the number of oocysts produced per mouse as well as prolong the shedding interval.
spacer
Citation:Miller, T. A., and F. W. Schaefer III. Use of Long Acting Steroid Methylprednisolone Acetate for the Prolongation of Cryptosporidium Sp. in Mice. Presented at VII International Workshops on Opportunistic Protists (IWOP), Cincinnati, OH, June 13-16, 2001.
spacer
spacer
Contact: Mary P. O'Bryant - (919)-541-4871 or obriant.mary@epa.gov
spacer
Division: Microbiological & Chemical Exposure Assessment Division
spacer
Branch: Biohazard Assessment Research Branch
spacer
Product Type: Abstrct/Oral
spacer
Presented: 06/13/2001
spacer
spacer

 

ORD Home | Search EPA | Search NERL | Search EIMS | Contacts | Help

 
Begin Site Footer

EPA Home | Privacy and Security Notice | Contact Us

Last Updated on Monday, October 22, 2007
URL: http://cfpub.epa.gov