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Development of a Molecular Method to Identify Hepatitis E Virus

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Abstract:Hepatitis E virus (HEV) is a waterborne emerging pathogen that causes significant illness in the developing world. Thus far, an HEV outbreak has not been reported in the U.S., although a swine variant of the virus is common in Midwestern hogs. Because viruses isolated from two U.S. cases of human hepatitis E were very similar to the swine strain, it may be that HEV is a zoonotic virus. It will therefore be important to determine to what extent this virus may be present in potable water supplies. We have developed a reverse transcription-polymerase chain reaction method that should be able to identify all of the known HEV strains. When evaluated under laboratory conditions, this assay has detected low levels of all HEV strains tested.
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Citation:Grimm, A. C., and G. S. Fout. Development of a Molecular Method to Identify Hepatitis E Virus. Presented at American Society for Virology, Ft. Collins, CO, July 8-12, 2000.
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Contact: Mary P. O'Bryant - (919)-541-4871 or obriant.mary@epa.gov
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Division: Microbiological & Chemical Exposure Assessment Division
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Branch: Biohazard Assessment Research Branch
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Product Type: Abstrct/Oral
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Presented: 07/12/2000
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Related Entries:
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Bullet Item Detecting Ccl-Related, Emerging Waterborne Human Viruses and Viral Indicators for Exposure Assessment
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Last Updated on Monday, October 22, 2007
URL: http://cfpub.epa.gov