Skip common site navigation and headers
United States Environmental Protection Agency
Exposure Research
Begin Hierarchical Links EPA Home > Research & Development > Exposure Research > Publications/Presentations > End Hierarchical Links

 

Rapid and Simplified Hplc Method With UV Detection, Ph Control and Selective Dechlorinator for Cyanuric Acid Analysis in Water

spacer
spacer
Abstract:Cyanuric acid (CA) and chloroisocyanurates are commonly used as standard ingredients in formulations for household bleaches, industrial cleansers, dishwasher compounds, general sanitizers, and chlorine stabilizers. They are very well known for preventing the photolytic decomposition of chlorine disinfectant. HPLC methods have been favored for CA analysis. However, striking variations in pH (1-10, most in the neutral range 6.7-7.8) and UV detection (196-225 nm) among these methods demand an operational method with pH control, practical sensitivity, and CA retention. This method is now fully developed as CA is measured adequately still using the HPLC-UV technique but with optimum parameters. UV spectra of CA show strong pH dependency with a strong 213 nm absorption at pHs>7. The HPLC setup combined a C18 separation column with 5% methanol/95% phosphate eluents over a wide array of pHs (2-10). Capacity factors were computed from 0.057 to 0.478. Practical sensitivity and intermediate retention were fond at pH 7.3. The complex equilibria of chlorinated isocyanurates in solution (up to 10 species) are eliminated to 2 species (CA and first ion) by dechlorination with ascorbic acid and watEr reaction resulting in chlorine liberation and CA product as confirmed by UV, HPLC-UV and LC-MS techniques. The proposed method exploits fundamental chemical equilibria, reactivity, and optical properties of CA to measure it in the practical 0.5-125 ug/ml range with an MDL of 0.05 ug/ml.
spacer
Citation:Cantu, R., O. M. Evans, and A. P. Dufour. Rapid and Simplified Hplc Method With UV Detection, Ph Control and Selective Dechlorinator for Cyanuric Acid Analysis in Water. Presented at American Chemical Society Regional Meeting, Covington, KY, May 16-19, 2000.
spacer
spacer
Contact: Mary P. O'Bryant - (919)-541-4871 or obriant.mary@epa.gov
spacer
Division: Microbiological & Chemical Exposure Assessment Division
spacer
Branch: Chemical Exposure Research Branch
spacer
Product Type: Abstrct/Oral
spacer
Presented: 05/16/2000
spacer
Related Entries:
spacer
Bullet Item Analytical Methods for Assessing the Magnitude of Human Ingestion of Recreational Water During Swimming Activity
spacer Relationship Reason:   A Project of the Product
spacer
spacer
spacer

 

ORD Home | Search EPA | Search NERL | Search EIMS | Contacts | Help

 
Begin Site Footer

EPA Home | Privacy and Security Notice | Contact Us

Last Updated on Monday, October 22, 2007
URL: http://cfpub.epa.gov