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Collecting Urine Samples from Young Children for Pesticide Studies

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Abstract:To estimate pesticide exposure for young children wearing diapers, a method for collecting urine samples for analysis of pesticide metabolites is needed. To find a practical method, two possibilities were investigated: (1) analysis of expressed urine from cotton diaper inserts and (2) analysis of the whole void extracted from the diaper (or other urine collection materials).

In the first study, the validity of using cotton gauze pads as diaper inserts to collect urine samples from young children was tested. Urine was spiked with a pesticide and four metabolites: 2,4-dichlorophenoxyacetic acid (which is mainly eliminated from urine unchanged), 3-phenoxybenzoic acid (a metabolite of synthetic pyrethroids), atrazine mercapturate (a metabolite of atrazine), malathion dicarboxylic acid (a metabolite of malathion) and 2-isopropyl-4-methyl-6-hydroxypyrimidine (a metabolite of diazinon). Aliquots of the spiked urine were added to the gauze pads and were expressed from the pads, using a syringe, immediately and after 1,2,4,8 and 24 hours to determine the impact of contact time on recovery. Expressed urine samples were then extracted and analyzed. The recoveries of target analytes from the expressed urine were within a range of 70-130%.

In the second study, potential urine collection materials such as cloth diaper, sanitary napkins, gel sorbers and clay were spiked with a list of 12 potential urinary metabolites of organophosphate and carbamate pesticides. The urine was extracted and analyzed. The recoveries for each tested material were determined and will be presented.
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Citation:Hu, Y., E. D. Pellizzari, J. H. Raymer, G. G. Akland, J. B. Beach, J. T. Keever, D. Barr, L. Needham, B. A. Schumacher, and L. J. Melnyk. Collecting Urine Samples from Young Children for Pesticide Studies. Presented at ISEA Annual Conference, Monterey, CA, October 24-27, 2000.
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Contact: Mary P. O'Bryant - (919)-541-4871 or obriant.mary@epa.gov
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Division: Microbiological & Chemical Exposure Assessment Division
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Branch: Chemical Exposure Research Branch
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Product Type: Abstrct/Oral
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Presented: 10/26/2000
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Related Entries:
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Bullet Item Dietary Intake of Young Children
spacer Relationship Reason:   A Project of the Product
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Last Updated on Monday, October 22, 2007
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