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Occurrence of Enteric Viruses in Surface Waters

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Abstract:Human enteric viruses cause a number of diseases when individuals are exposed to contaminated drinking & recreational waters. Vaccination against poliovirus has virtually eliminated poliomyelitis from the planet. Other members of enterovirus group cause numerous diseases. Hepatitis A and E have caused large waterborne hepatitis outbreaks. 2nd leading cause of illness in U.S. is acute nonbacterial gastroenteritis, which results from infection of suseptible individuals with members of caliciviridae, astroviridae, reviridae & adenoviridae families. First step in establishing risk of waterborne disease from these viruses is to determine levels of occurrence in contaminated waters. This requires methods to recover, identify and measure concentration of viruses in affected waters. Rapid polymerase chain reaction (PCR) methods have been developed to measure virus levels in environmental water. This study was performed to determine whether a multiplex PCR method developed to characterize the occurrence of enteroviruses, reoviruses, rotaviruses, hepatitis A and Norwalk virus in groundwater could be used iwth surface waters. The method was tested using surface waters from 5 USGS NAWQA Program sites selected to provide good geographic coverage of the U.S. and to span a range of hydroclimatic and land-use settings. All sites were found to be positive for two or more of the virus groups during the course of the study. The PCR findings from the NAWQA sites will be used to illustrate these problems and the proper interpretation of PCR-positive results.
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Citation:Fout, G. S. Occurrence of Enteric Viruses in Surface Waters. Presented at USGS Workshop "Building Capabilities for Monitoring & Assessment of Public Health Microbiology, Columbus, OH, March 14-16, 2000.
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Contact: Mary P. O'Bryant - (919)-541-4871 or obriant.mary@epa.gov
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Division: Microbiological & Chemical Exposure Assessment Division
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Branch: Biohazard Assessment Research Branch
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Product Type: Abstrct/Oral
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Presented: 03/14/2000
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Related Entries:
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Bullet Item Measure Occurrence and Exposure to Waterborne Human Viruses
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Last Updated on Monday, October 22, 2007
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