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Detection of Infectious Adenovirus in Tertiary Treated and UV Disinfected Wastewater During a UV Disinfection Pilot Study

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Abstract:An infectious enteric adenovirus was isolated from urban wastewater receiving tertiary treatment and ultraviolet (UV) disinfection. A pilot study was undertaken to investigate the efficacy of UV disinfection (low pressure, high intensity radiation) of total and fecal coliform bacteria, naturally occurring male-specific coliphage, seeded male-specific coliphage (MS20, naturally occurring enteirc viruses, and seeded poliovirus type 1 ((Lsc) in tertiary treated wastewater. The enteric viruses were concentrated from ca. 400 L of UV treated (20-70 mW-sec/cm2 doses) effluent using traditional adsorption elution methods and detected via a tissue culture cytopathic effects (CPE) assay. Sample concentrates were applied to monolayers of the CaCo-2 cell line and observed for a minimum of 14 days for CPE, followed by a minimum of 2 succeeding passages. Analysis of the UV treated samples for natural enteric viruses revealed the presence of a fastidious CPE-producing virus, later identified as an adenovirus by electron microscopic analysis. The adenovirus was isolated from a sample receiving a UV dose (ca. 60 mW-s/cm2) which reduced total and fecal coliform levels by ca. 5 Log10 units. Pilot unit data further showed that the same dose was capable of removing ca. 4 Log10 units of seeded MS2 coliphage. These results suggest that while UV disinfection may be effective at reducing levels of indicator bacteria, natural and seeded bacteriophage, and even seeded poliovirus, it may not provide equivalent reductions of some naturally occurring infectious enteric viruses such as adenovirus. Further research is need to determine appropriate doses for UV disinfection of enteric viruses in wastewater.
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Citation:Thompson, S., J. L. Jackson, M. Suvacastillo, W. A. Yanko, and F. P. Williams Jr. Detection of Infectious Adenovirus in Tertiary Treated and UV Disinfected Wastewater During a UV Disinfection Pilot Study. Presented at WEFTEC 2000, 73rd Annual Conference & Exposition, Anaheim, CA, October 14-18, 2000.
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Contact: Mary P. O'Bryant - (919)-541-4871 or obriant.mary@epa.gov
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Division: Microbiological & Chemical Exposure Assessment Division
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Branch: Biohazard Assessment Research Branch
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Product Type: Abstrct/Oral
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Presented: 10/15/2000
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Related Entries:
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Bullet Item Measure Occurrence and Exposure to Waterborne Human Viruses
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Last Updated on Monday, October 22, 2007
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