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Testing Methods for Detection of Cryptosporidium Spp. in Water Samples

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Abstract:A large waterborne outbreak of cryptosporidiosis in Milwaukee, Wisconsin, U.S.A. in 1993 prompted a search for ways to prevent large scale waterborne outbreaks of protozoan parasitoses. Two principle strategies have emerged: risk assessment leading to appropriate treatment and pathogen monitoring. Methods for detecting C. parvum play an integral role in both strategies.

One criticism of current methods for detecting protozoa in water is that they produce results that are highly variable. It is difficult to determine if the methods themselves, or the procedures for testing them, are the source of the variability. If testing procedures are responsible for high variability, then the way in which methods are tested may not provide a fair comparison.

To overcome this problem, the U.S. EPA has developed a set of criteria to aid in the evaluation of detection methods for parasites in water. Application of these criteria has elucidated some of the most important causes of testing variability. As a consequence, the Agency has developed approaches to overcome these pitfalls. The results from comparing several of these methods indicate that a monitoring strategy is not optimal at this time.
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Citation:Lindquist, H. A., M. W. Ware, R. E. Stetler, and F. W. Schaefer III. Testing Methods for Detection of Cryptosporidium Spp. in Water Samples. Presented at 3rd Seminar on Food and Waterborne Parasitic Zoonoses in the 21th Century, Bangkok, Thailand, December 6-8, 2000.
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Contact: Mary P. O'Bryant - (919)-541-4871 or obriant.mary@epa.gov
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Division: Microbiological & Chemical Exposure Assessment Division
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Branch: Biohazard Assessment Research Branch
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Product Type: Abstrct/Oral
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Presented: 12/07/2000
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Related Entries:
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Bullet Item Detecting Ccl-Related, Emerging and Regulated Waterborne Human Protozoa for Exposure Assessment
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Last Updated on Monday, October 22, 2007
URL: http://cfpub.epa.gov