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Nitro Musk Bound to Carp Hemoglobin: Determination By Gc With Two MS Detection Modes: Eims Versus Electron Capture Negeative Ion MS

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Abstract:Nitroaromatic compounds including synthetic nitro musks are important raw materials and intermediates in the synthesis of explosives, dyes, and pesticides, pharmaceutical and personal care-products (PPCPs). The nitro musks such as musk xylene (MX) and musk ketone (MK) are extensively used as fragrance ingredients in PPCPs and other commercial toiletries. Identification and quantification of a bound 4-amino-MX (4-AMX) metabolite as well as a 2- amino-MK (2-AMK) metabolite were carried out by gas chromatography-mass spectrometry' (GC/MS), with selected ion monitoring (SIM) in both the electron ionization (ElMS) and electron capture (EC) negative ion chemical ionization (NICIMS) modes. Detection of 4-AMX and 2-AMK occurred after the cysteine adducts in carp hemoglobin, derived from the nitroso metabolites, were released by alkaline hydrolysis. The released metabolites were extracted into n-hexane. The extract was preconcentrated by evaporation, and analyzed by GC-SIM-MS. A comparison between the El and EC approaches was made. EC NICIMS detected both metabolites whereas only 4-AMX was detected by ElMS. The EC NICIMS approach exhibited fewer matrix responses and provided a lower detection limit. Quantitation in both approaches was based on internal standard and a calibration plot.
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Citation:Mottaleb, M. A., W. C. Brumley, and G. W. Sovocool. Nitro Musk Bound to Carp Hemoglobin: Determination By Gc With Two MS Detection Modes: Eims Versus Electron Capture Negeative Ion MS. INTERNATIONAL JOURNAL OF ENVIRONMENTAL ANALYTICAL CHEMISTRY. Taylor & Francis, Inc., Philadelphia, PA, 84(14 & 15):1069-1078, (2004).
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Contact: Chris Siebert - (702) 798-2234 or siebert.christopher@epa.gov
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Division: Environmental Sciences Division
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Branch: Environmental Chemistry Branch
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Product Type: Journal
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Published: 12/22/2004
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Downloads:
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Bullet Item Comparison of Determining Bound Nitro Musk Metabolites in Carp Hemoglobin Using Eims and Electron Capture Negative Ion MS
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