Skip common site navigation and headers
United States Environmental Protection Agency
Exposure Research
Begin Hierarchical Links EPA Home > Research & Development > Exposure Research > Publications/Presentations > End Hierarchical Links

 

Comparison of Enterococcus Measurements in Freshwater at Two Recreational Beaches By Quantitative Polymerase Chain Reaction and Membrane Filer Culture Analysis

spacer
spacer
Abstract:Cell densities of the fecal pollution indicator genus, Enterococcus, were determined by a rapid (2-3 hr) quantitative PCR (QPCR) analysis based method in 100 ml water samples collected from recreational beaches on Lake Michigan and Lake Erie during the summer of 2003. Enumeration results by this method were compared with counts of Enterococcus.colony forming units (CFU) determined by filter plating on mEI-agar selective medium. The QPCR method showed a 95% confidence, minimum detection limit of approximately two Enterococcus cells per sample in analyses of undiluted DNA extracts and highly accurate quantitation of spiked lake water samples based on the geometric means of replicate sample results. Geometric means of Enterococcus densities, determined from multiple collection points during each sampling visit, showed approximately lognormal distributions at both beaches using both QPCR and culture analyses. These geometric means ranged from 10 to 8548 cells by QPCR analysis and 1 to 2499 CFU by culture analysis in Lake Michigan (N = 56) and from 8 to 8695 cells by QPCR analysis and 3 to 1941 CFU by culture analysis in Lake Erie (N = 47). Regression analysis of these results showed a high level of agreement between the two methods with an overall correlation coefficient of 0.68.
spacer
Citation:Haugland, R. A., S. D. Siefring, L. J. Wymer, K. Brenner, and A. P. Dufour. Comparison of Enterococcus Measurements in Freshwater at Two Recreational Beaches By Quantitative Polymerase Chain Reaction and Membrane Filer Culture Analysis. WATER RESEARCH 39(4):559-568, (2005).
spacer
spacer
Contact: Mary P. O'Bryant - (919)-541-4871 or obriant.mary@epa.gov
spacer
Division: Microbiological & Chemical Exposure Assessment Division
spacer
Branch: Microbial Exposure Research Branch
spacer
Product Type: Journal
spacer
Published: 02/01/2005
spacer
Downloads:
spacer
Bullet Item Comparison of Enterococcus Measurements in Freshwater at Two Recreational Beaches By Quantitative Polymerase Chain Reaction and Membrane Filer Cultural Analysis
spacer
spacer
Related Entries:
spacer
Bullet Item Measurements of Fecal Contamination in Recreational Waters By New Rapid Methods and Their Relationships to Health Endpoints
spacer Relationship Reason:   A Project of the Product
spacer
spacer
spacer

 

ORD Home | Search EPA | Search NERL | Search EIMS | Contacts | Help

 
Begin Site Footer

EPA Home | Privacy and Security Notice | Contact Us

Last Updated on Monday, October 22, 2007
URL: http://cfpub.epa.gov