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Immunoassay Methods for Measuring Atrazine and 3,5,6-Trichloro-2-Pyridinol in Foods

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Abstract:This chapter describes the use of enzyme-linked immunosorbent assay (ELISA) methods for the analysis of two potential environmental contaminants in food sample media, atrazine and 3,5,6-trichloro-2-pyridinol (3,5,6-TCP). Two different immunoassay formats are employed: a magnetic-particle immunoassay testing kit and a 96-microwell plate immunoassay. Diluted, filtered, nonfat baby foods were analyzed directly by a commercial magnetic-particle immunoassay testing kit for the determination of atrazine. Fatty baby foods were extracted with water for the magnetic-particle immunoassay analysis of obtrusion. For 3,5,6-TCP analysis, the food samples from a duplicate diet exposure study were analyzed by a laboratory-based 96-micro well plate immunoassay. Acidic methanol (72% methanol, 26% water, and 2% acetic acid) was the solvent for the food samples.

The United States Environmental Protection agency (EPA) through its Office of Research and Development funded and collaborated in the research described here under contract No.68-D-99-011 to Battelle. It has been subjected to Agency review and approved for publication. Mention of trade names or commercial products does not constitute endorsement or recommendation by the EPA for use.
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Citation:Van Emon, J. M., and J. C. Chuang. Immunoassay Methods for Measuring Atrazine and 3,5,6-Trichloro-2-Pyridinol in Foods. Methods in Biotechnology, Chapter18. Methods in Biotechnology. Humana Press Incorporated, Totowa, NJ, 19:231-238, (2006).
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Contact: Liz Hope - (919) 541-2785 or hope.elizabeth@epa.gov
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Division: Human Exposure & Atmospheric Sciences Division
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Branch: Exposure & Dose Research Branch
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Product Type: Book Chaptr
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Published: 11/01/2005
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Downloads:
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Bullet Item Immunoassay Methods for Measuring Atrazine and 3,5,6-Trichloro-2-Pyridinol in Foods
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Related Entries:
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Bullet Item Human Exposure Measurements/Methods: Develop and Test Immunochemical and Other Screening Techniques for Use in Exposure Monitoring
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Last Updated on Monday, October 22, 2007
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