OLS : Record


Main Title The Dynamics of an estuary as a natural ecosystem, II /
Author Koch, Robert B.; Vernberg, F. J.; Kitchens, W.; McKellar, H.; Summers, K.; Bonnell, R.
CORP Author Mississippi State Univ., Mississippi State. Dept. of Biochemistry.;Environmental Research Lab., Gulf Breeze, FL.
Publisher U.S. Environmental Protection Agency, Office of Research and Development, Environmental Research Laboratory ; National Technical Information Service [distributor],
Place Published Gulf Breeze, Fla. Springfield, Va.
Year Published 1978
Report Number EPA-600/3-78-092; EPA-R-804407-01
Stock Number PB-289 969
OCLC Number 08081667
Subjects Pesticides; Antibodies; Development; Chlorine organic compounds; Inhibition; Recommendations; Rabbits; Chickens; Laboratory animals; Antigen antibody reactions; Enzymes; Insecticides; Chlorine organic compounds; Kelevan; Adenosine triphosphatase; Kepone; Immune serums; Methenocyclobutapentalene levulinic acid/decachlorooctahydro-hydroxy-(ethyl-ester); Cyclobutapentalenone/decachlorocathydro; North Inlet Estuary; Ecology; Water pollution; Mathematical models; Aquatic plants; Aquatic animals; Plankton; Intertidal zone; Marine microorganisms; Invertebrates; Food chains; Primary biological productivity; Fishes; Birds; Respiration; Biomass; Nutrients; Computer programming; South Carolina; Spartina alterniflora; Salt marshes; Baseline studies
Subject Added Ent Estuarine ecology--South Carolina.; Estuarine ecology--Mathematical models.; Salt marsh ecology--South Carolina.; Salt marsh ecology--Mathematical models.; Tidemarsh ecology--South Carolina; Tidemarsh ecology--Mathematical models
Internet Access
Description Access URL
Library   Call Number Additional Info Location Date Modified
EIAD EPA-600/3-78-092 Region 2 Library/New York,NY 05/11/2005
EJAD EPA 600/3-78-092 Region 3 Library/Philadelphia, PA 03/19/1993
EJBD ARCHIVE EPA 600-3-78-092 Headquarters Library/Washington,DC 11/13/2013
EJBD EPA 600-3-78-092 c.1 Headquarters Library/Washington,DC 03/24/2014
EJDD EPA 600/3-78-092 OASQA Library/Fort Meade,MD 03/31/1995
EKAM QP82.2.P6E2 600, 1978, no.092 Region 4 Library/Atlanta,GA 05/13/1994
EKCD EPA-600/3-78-092 NHEERL/GED Library/Gulf Breeze,FL 07/21/2015
EOAD EPA 600/3-78-092 Region 8 Technical Library/Denver,CO 02/28/2011
ERAD EPA 600/3-78-092 Region 9 Library/San Francisco,CA 10/22/2012
NTIS PB-289 963 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 06/23/1988
NTIS PB-289 969 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 06/23/1988
Collation viii, 76 pages : illustrations ; 28 cm.
Abstract The report describes development of an antibody to an organochlorine pesticide to be used in studies related to its inhibition of the ATPase system. Kelevan, the condensation product of ethyl levulinate and Kepone, was successfully conjugated to bovine serum albumin (BSA), fibrinogen (BF), and gamma globulin (BGG). Rabbits and chickens preimmunized with BSA and then immunized with BSA-Kelevan produced antibodies to both the hapten, Kelevan, and the carrier protein BSA. Antiserum to Kelevan protected ATPase activity against Kepone and its derivatives. The titer of antibody to Kelevan was critical since antiserum with only trace amounts of Kelevan antibody failed to protect the ATPase activity against Kepone Inhibition. Antibody was concentrated by Na2S04 fractional precipitation of the antiserum and obtained in pure form by affinity chromatography using BGG-Kel covalently linked to Sepharose 4B. Pure antibody was obtained from untreated blood serum or plasma with no prior pretreatment or fractionation using the BGG-Kel affinity column. Complete protection of mitochondrial mg2+ATPase activity from in vitro inhibition of Kepone was obtained using a 1.2 mg quantity of na2S04 fractionated antibody and only 120 micrograms of pure antibody. Reversal of ATPase inhibition was readily obtained by addition of antibody prior to addition of substrate to the reaction mixture.
Notes "Belle W. Baruch Institute for Marine Biology and Coastal Research, University of South Carolina. "October 1978." Includes bibliographical references (pages 72-76).
Supplementary Notes See also PB-271 357.
Author Added Ent
Vernberg, F. John,
Corporate Au Added Ent Environmental Research Laboratory (Gulf Breeze, Fla.); Belle W. Baruch Institute for Marine Biology and Coastal Research.
Title Ser Add Ent Research reporting series. Ecological research ; EPA-600/3-78-092.
NTIS Title Notes Final rept. 1 Mar 76-28 Feb 78.
PUB Date Free Form 1978
Series Title Traced Research reporting series Ecological research ; EPA-600/3-78-092
Series Title Untraced Research reporting series ; EPA-600/3-78-092
Category Codes 8A; 6F; 13B; 47D; 57H; 68D
NTIS Prices PC A05/MF A01
Primary Description 600/04
BIB Level m
Medium unmediated
Control Number 323754442
OCLC Time Stamp 20150506164159
Cataloging Source OCLC/T
Language eng
SUDOCS Number EP 1.23:600/3-78-092
Origin OCLC
OCLC Rec Leader 02521cam 2200505Ia 45020