| Main Title |
Vibrio cholerae 01 Can Assume a 'Rugose' Survival Form That Resists Killing by Chlorine, Yet Retains Virulence. |
| Author |
Rice, E. W.;
Johnson, C. H.;
Clark, R. M.;
Fox, K. R.;
Reasoner, D. J.;
|
| CORP Author |
Environmental Protection Agency, Cincinnati, OH. Risk Reduction Engineering Lab. ;Maryland Univ. at Baltimore. School of Medicine.;Department of Veterans Affairs Medical Center, Baltimore, MD.;Public Health Service, Rockville, MD. |
| Publisher |
c1993 |
| Year Published |
1993 |
| Report Number |
EPA/600/J-93/381; PHS-R01A128856; |
| Stock Number |
PB94-117504 |
| Subjects |
Vibrio cholerae;
Chlorine;
Virulence;
Cultured cells;
Phenotype;
Microbial colony count;
Microbial sensitivity tests;
Rabbits;
Bacterial adhesion;
pH;
Reprints;
|
| Holdings |
| Library |
|
Call Number |
Additional Info |
Location |
Date Modified |
| NTIS |
|
PB94-117504 |
Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. |
NTIS |
02/27/1994 |
|
| Collation |
10p |
| Abstract |
Vibrio cholerae 01 is able to shift between smooth and rugose colonial morphologies. Cultures of smooth V. cholerae strains were inactivated in less than 20 s at a concentration of 1.0 mg/l free chlorine. In contrast, cultures of rugose variants exposed to this concentration of chlorine showed an initial rapid drop in viable counts, followed by persistence of a protected subpopulation of cells. Viable V. cholerae could still be recovered from rugose cultures even after exposure to 2.0 mg/l free chlorine for 30 min. Preliminary studies suggest that resistance to killing by chlorine was due to formation of cell aggregates enclosed in a gelatinous mucoid material. Rugose strains appeared to be fully virulent, based on their ability to adhere to Caco-2 cells and elicit fluid accumulation in rabbit ileal loops. Our data suggest that the V. cholerae rugose phenotype represents a fully virulent survival form of the organism that can persist in the presence of free chlorine. (Copyright (c) 1993 Chapman & Hall.) |
| Supplementary Notes |
Pub. in Jnl. of Health Research, v3 p89-98 1993. Prepared in cooperation with Maryland Univ. at Baltimore. School of Medicine. Sponsored by Department of Veterans Affairs Medical Center, Baltimore, MD., and Public Health Service, Rockville, MD. |
| NTIS Title Notes |
Journal article. |
| Title Annotations |
Reprint: Vibrio cholerae 01 Can Assume a 'Rugose' Survival Form That Resists Killing by Chlorine, Yet Retains Virulence. |
| Category Codes |
57K; 57Y |
| NTIS Prices |
PC A02/MF A01 |
| Primary Description |
600/14 |
| Document Type |
NT |
| Control Number |
400732864 |
| Cataloging Source |
NTIS/MT |
| Origin |
NTIS |
| Type |
CAT |
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