| Main Title |
Determination of Acrylamide in Rat Serum and Sciatic Nerve by Gas Chromatography-Electron-Capture Detection. |
| Author |
Raymer, J. H.;
Sparacino, C. M.;
Velez, G. R.;
Padilla, S.;
MacPhail, R. C.;
|
| CORP Author |
Health Effects Research Lab., Research Triangle Park, NC. Neurotoxicology Div. ;Research Triangle Inst., Research Triangle Park, NC. Analytical and Chemical Sciences Unit. |
| Publisher |
c1993 |
| Year Published |
1993 |
| Report Number |
EPA/600/J-94/070; |
| Stock Number |
PB94-141587 |
| Subjects |
Blood;
Sciatic nerve;
Acrylamides;
Chemical analysis;
Gas chromatography;
Electrons;
Kinetics;
Tissues(Biology);
Mass spectroscopy;
Dose-response relationships;
Reprints;
|
| Holdings |
| Library |
|
Call Number |
Additional Info |
Location |
Date Modified |
| NTIS |
|
PB94-141587 |
Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. |
NTIS |
05/14/1994 |
|
| Collation |
14p |
| Abstract |
A modified method for the derivatization and determination of acrylamide as 2-bromopropenamide by gas chromatography-electron-capture detection was developed and applied to serum and sciatic nerve from rats. The method was accurate and precise over the calibration range 2.24-7.47 micrograms/ml in serum diluted 1:125 and 4-122 micrograms/g in sciatic nerve homogenate (5 mg/ml). Limits of detection were estimated to be 1,200 ng/ml in undiluted serum and 3 micrograms/g in intact sciatic nerve. The use of less dilute samples to allow for lower limits of detection appears feasible. The time-course of acrylamide in serum and sciatic nerve was studied after acute dosing and indicated elimination half-lives of 1.8 and 2.0 h for serum and sciatic nerve, respectively. A dose-effect relationship was established for each matrix after acute dosing and the measured acrylamide concentrations in serum (micrograms/ml) were approximately the same as in sciatic nerve (micrograms/g). (Copyright (c) 1993 Elsevier Science Publishers B.V.) |
| Supplementary Notes |
Pub. in Jnl. of Chromatography 619, n2 p223-234 29 Oct 93. Prepared in cooperation with Research Triangle Inst., Research Triangle Park, NC. Analytical and Chemical Sciences Unit. |
| NTIS Title Notes |
Journal article. |
| Title Annotations |
Reprint: Determination of Acrylamide in Rat Serum and Sciatic Nerve by Gas Chromatography-Electron-Capture Detection. |
| Category Codes |
99A |
| NTIS Prices |
PC A03/MF A01 |
| Primary Description |
600/10 |
| Document Type |
NT |
| Control Number |
407722629 |
| Cataloging Source |
NTIS/MT |
| Origin |
NTIS |
| Type |
CAT |
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