OLS : Record

RECORD NUMBER: 37 OF 71

Main Title Morphological Transformation and DNA Adduct Formation by Benz(j)aceanthrylene and Its Metabolites in C3H10T1/2CL8 Cells: Evidence for Both Cyclopenta-Ring and Bay-Region Metabolic Activation Pathways.
Author Nesnow, S.; Lasley, J.; Curti, S.; Ross, J.; Nelson, G.;
CORP Author Environmental Health Research and Testing, Inc., Research Triangle Park, NC. ;North Carolina Univ. at Chapel Hill. Dept. of Environmental Sciences and Engineering.;Environmental Protection Agency, Research Triangle Park, NC. Carcinogenesis and Metabolism Branch.
Publisher c1991
Year Published 1991
Report Number EPA-68-02 4456; EPA/600/J-92/062;
Stock Number PB92-150762
Subjects Mutagens; DNA adducts; Metabolism; Aromatic polycyclic hydrocarbons; Cultured cells; Metabolic activation; Phosphorus 32; Cell survival; Cell morphology; Thin layer chromatography; Reprints;
Holdings
Library   Call Number Additional Info Location Date Modified
NTIS PB92-150762 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 08/28/1992
Collation 8p
Abstract Benz(j)aceanthrylene (B(j)A), a cyclopenta-fused polycyclic aromatic hydrocarbon related to 3-methylcholanthrene, has been studied to identify the major routes of metabolic activation in transformable C3H10T1/2CL8(C3H10T1/2) mouse embryo fibroblasts in culture. Previous studies have reported that the major (55% of total) B(j)A metabolite formed by C3H10T1/2 cells was (+ or -)-trans-9,10-dihydroxy-B(j)A(B(jA-9,10-diol), the dihydrodiol in the bay-region ring, with moderate amounts (14% of total) of (+ or -)-trans-1,2-dihydro-1,2-dihydroxy-B(j)A (B(j)A-1,2-diol), the cyclopenta-ring dihydrodiol. Qualitative and quantitative analysis of the postlabeling data suggests that B(j)A is metabolically activated by two distinct routes, the bay-region diol-epoxide route and the cyclopenta-ring oxide route, the former being the most significant.
Supplementary Notes Pub. in Jnl. of Cancer Research, v51 p6163-6169, 15 Nov 91. See also PB85-124709, PB89-109797 and PB90-103458. Prepared in cooperation with North Carolina Univ. at Chapel Hill. Dept. of Environmental Sciences and Engineering. Sponsored by Environmental Protection Agency, Research Triangle Park, NC. Carcinogenesis and Metabolism Branch.
NTIS Title Notes Journal article.
Title Annotations Reprint: Morphological Transformation and DNA Adduct Formation by Benz(j)aceanthrylene and Its Metabolites in C3H10T1/2CL8 Cells: Evidence for Both Cyclopenta-Ring and Bay-Region Metabolic Activation Pathways.
Category Codes 57Y; 57F; 57B
NTIS Prices PC A02/MF A01
Primary Description 600/10
Document Type NT
Control Number 211522896
Cataloging Source NTIS/MT
Origin NTIS
Type CAT