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RECORD NUMBER: 25 OF 74

Main Title Ki-ras Oncogene Mutations in Tumors and DNA Adducts Formed by Benz(i)aceanthrylene and Benzo(a)pyrene in the Lungs of Strain A/J Mice.
Author Mass, M. J.; Jeffers, A. J.; Ross, J. A.; Nelson, G.; Galati, A. J.;
CORP Author Environmental Health Research and Testing, Inc., Research Triangle Park, NC. ;Medical Coll. of Ohio at Toledo. Dept. of Pathology. ;Ohio State Univ., Columbus. Arthur James Cancer Hospital.;Health Effects Research Lab., Research Triangle Park, NC. Genetic Toxicology Div.
Publisher c1993
Year Published 1993
Report Number EPA/600/J-94/066;
Stock Number PB94-139557
Subjects Ras genes; Mutation; Neoplasms; DNA adducts; Benzo(a)pyrene; Lung; Toxicology; Codon; Mice; DNA sequence analysis; Deoxyribonucleic acids; Reprints; Benz(j)aceanthrylene
Holdings
Library   Call Number Additional Info Location Date Modified
NTIS PB94-139557 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 05/14/1994
Collation 8p
Abstract Strain A/J mice received intraperitoneal injections of benz(j)aceanthrylene(B(j)A) or benzo(a)pyrene (B(a)P). At 24, 48, and 72 h, lung tissues were removed for analysis of B(a)P- or B(j)A-derived DNA adduct formation during the first 3 d of exposure. One group of mice exposed to these hydrocarbons was kept for 8 mo to determine lung tumor multiplicity, the occurrence of mutations in codons 12 and 61 of the Ki-ras gene in the tumors that arose, the relationship between Ki-ras oncogene mutations in tumors, and the presence and quantity of genomic DNA adducts. Analysis of adduction patterns in DNA suggested that B(j)A was activated to form DNA-binding derivatives in A/J mouse lungs primarily at the cyclopenta ring even though B(j)A contains a bay region. As reported in the published literature, the mutation spectrum induced by 3-MCA in Ki-ras codon 12 of mouse cells is similar to that of B(a)P but not to that of its close relative B(j)A. In contrast to B(j)A, 3-MCA is activated mostly via a bay-region diol-epoxide since its cyclopenta ring is saturated and not easily epoxidated. Therefore, we propose that the GGT --> CGT mutations produced by B(j)A in Ki-ras codon 12 were mostly the result of cyclopenta-ring-derived adducts.
Supplementary Notes Pub. in Molecular Carcinogenesis 8, n3 p186-192 Nov 93. Prepared in cooperation with Medical Coll. of Ohio at Toledo. Dept. of Pathology, and Ohio State Univ., Columbus. Arthur James Cancer Hospital. Sponsored by Health Effects Research Lab., Research Triangle Park, NC. Genetic Toxicology Div.
NTIS Title Notes Journal article.
Title Annotations Reprint: Ki-ras Oncogene Mutations in Tumors and DNA Adducts Formed by Benz(i)aceanthrylene and Benzo(a)pyrene in the Lungs of Strain A/J Mice.
Category Codes 57Y; 57F
NTIS Prices PC A02/MF A01
Primary Description 600/10
Document Type NT
Control Number 407721737
Cataloging Source NTIS/MT
Origin NTIS
Type CAT