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RECORD NUMBER: 41 OF 142

Main Title Genotoxicity of 2-Amino-6-N-Hydroxyadenine (AHA) to Mouse Lymphoma and CHO Cells.
Author Moore, M. M.; Harrington-Brock, K.; Parker, L.; Doerr, C. L.; Hozier, J. C.;
CORP Author Environmental Health Research and Testing, Inc., Research Triangle Park, NC. ;Florida Inst. of Tech., Melbourne. Medical Genetics Lab.;Health Effects Research Lab., Research Triangle Park, NC. Genetic Toxicology Div.
Publisher c1991
Year Published 1991
Report Number EPA-68-02-4456; EPA/600/J-91/213;
Stock Number PB91-243048
Subjects Mutagens; Lymphoma; Mutation; Mice; Chinese hamsters; Thymidine kinase; Chromosome aberrations; Micronucleus tests; Karyotyping; Hypoxanthine phosphoribosyltransferase; Ouabain; Chromosome mapping; Reprints; Aminohydroxyadenines
Holdings
Library   Call Number Additional Info Location Date Modified
NTIS PB91-243048 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 11/26/1991
Collation 11p
Abstract 2-Amino-6-N-hydroxyadenine (AHA) treated L5178Y/TK(+/-)-3.7.2C mouse lymphoma cells were evaluated for mutations at the tk, hgprt, and Na(1+)/K(1+) ATPase loci, as well as for gross chromosome aberrations and induction of micronuclei. In addition, AHA was evaluated for its ability to induce HGPRT mutants in CHO cells. AHA was found to induce mutations at all evaluated loci and in both cell types. The TK mutants were primarily large colonies although a few small colonies were also induced, particularly at the higher concentrations. Preliminary cytogenetic analysis of AHA-treated mouse lymphoma cells indicated that some gross aberrations but not micronuclei were induced. The 20 small-colony TK mutants evaluated by banded karyotype indicate that only a small fraction (2 of 20) showed chromosome 11 abnormalities. From these studies, it appears that AHA may be one of a very few chemicals that is capable of inducing multi-locus point mutations, with only slight clastogenic activity. Particularly at the higher concentrations, some of the mutants may contain multi-locus point mutations that result in slow growth. (Copyright (c) 1991 Elsevier Science Publishers B.V.)
Supplementary Notes Pub. in Mutation Research, v253 p63-71 1991. Prepared in cooperation with Florida Inst. of Tech., Melbourne. Medical Genetics Lab. Sponsored by Health Effects Research Lab., Research Triangle Park, NC. Genetic Toxicology Div.
NTIS Title Notes Journal article.
Title Annotations Reprint: Genotoxicity of 2-Amino-6-N-Hydroxyadenine (AHA) to Mouse Lymphoma and CHO Cells.
Category Codes 57Y; 57F
NTIS Prices PC A03/MF A01
Primary Description 600/10
Document Type NT
Control Number 131122060
Cataloging Source NTIS/MT
Origin NTIS
Type CAT