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RECORD NUMBER: 36 OF 142

Main Title V(D)J Recombinase-Mediated Deletion of the 'hprt' Gene in T-Lymphocytes from Adult Humans.
Author Fuscoe, J. C.; Zimmerman, L. J.; Harrington-Brock, K.; Burnette, L.; Moore, M. M.;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. ;Environmental Health Research and Testing, Inc., Research Triangle Park, NC. ;Vermont Univ., Burlington. Genetics Lab.
Publisher c1992
Year Published 1992
Report Number EPA/600/J-92/422;
Stock Number PB93-141216
Subjects Hypoxanthine phosphoribosyltransferase; T-lymphocyte gene rearrangement; Mutagenesis; Chromosome deletion; Base sequence; DNA mutational analysis; Mutations; Gene expression; Exons; Lymphocytes; Polymerase chain reaction; Southern blotting; Adults; Clone cells; Newborns; Reprints; V(D)J recombinase
Holdings
Library   Call Number Additional Info Location Date Modified
NTIS PB93-141216 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 06/12/1993
Collation 10p
Abstract The hprt T-cell cloning assay allows the detection of mutations occurring in vivo in the hypoxanthine guanine phosphoribosyltransferase (hprt) gene of T-lymphocytes. In the report, we examined a collection of 314 hprt-deficient clones derived from adult humans for evidence that the mutations were caused by the illegitimate activity of V(D)J recombinase by analyzing exons 2+3 deletion mutations. DNA sequence analysis of deletion breakpoint junctions showed that eight of the mutations were the result of V(D)J recombinase activity. The frequency of the recombinase-mediated mutations was similar in the adults and newborns (2-4 x 10 to the power of -7). Unregulated expression of V(D)J recombinase activity may be an important mechanism for genomic rearrangements in the genesis of cancer.
Supplementary Notes Pub. in Mutation Research, v283 n1 p13-20 Sep 92. Prepared in cooperation with Environmental Health Research and Testing, Inc., Research Triangle Park, NC., and Vermont Univ., Burlington. Genetics Lab.
NTIS Title Notes Journal article.
Title Annotations Reprint: V(D)J Recombinase-Mediated Deletion of the 'hprt' Gene in T-Lymphocytes from Adult Humans.
Category Codes 57F
NTIS Prices PC A02/MF A01
Primary Description 600/10
Document Type NT
Control Number 307031280
Cataloging Source NTIS/MT
Origin NTIS
Type CAT


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