OLS : Record

RECORD NUMBER: 17 OF 19

Main Title Utilization of Trifluorothymidine (TFT) to Select for Thymidine Kinase-Deficient Mutants from L5178Y/TK Mouse Lymphoma Cells.
Author Moore-Brown, M. M.; Clive, D.; Howard, B. E.; Batson, A. G.; Johnson, K. O.;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. ;Burroughs Wellcome Co., Research Triangle Park, NC. ;North Carolina Univ. at Chapel Hill.
Year Published 1981
Report Number EPA-600/J-81-687;
Stock Number PB84-174515
Subjects Toxicology; Mice; Cells(Biology); Reprints; Thymidine/trifluoro; Mutagenesis
Holdings
Library   Call Number Additional Info Location Date Modified
NTIS PB84-174515 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 06/23/1988
Collation 18p
Abstract Trifluorothymidine (TFT), a thymidine analog, was analyzed for its ability to select for thymidine kinase-deficient (TK-/-) mutants. In comparison with BUdR, the traditional selective agent for TK-/- cells, it was determined that TFT at 1/50th the dose (1 micrograms/ml vs. 50 micrograms/ml) is a more effective and versatile selective agent for TK-/- mutants arising from the TKt/- 3.7.2C heterozygote of L5178Y mouse lymphoma cells. Since TFT acts more rapidly than BUdR, it can be utilized in procedures (such as the analysis of the phenotypic lag) requiring the fast arrest of cell division. Reconstruction analyses of effective TK-/- mutant recovery indicate that TFT can be used to recover mutants from significantly higher densities of TIt/- cells than can BUdR. In addition, TK -/- mutants can attain larger colony size in TFT than in BUdR where severe stunting of growth occurs at high TK-/- cell densities. 190 of 194 isolated TFT-resistant large and small colony mutants (both spontaneous and induced).
Availability Notes Pub. in Mutation Research 85, p363-378 1981. Prepared in cooperation with Burroughs Wellcome Co., Research Triangle Park, NC., and North Carolina Univ. at Chapel Hill.
NTIS Title Notes Journal article.
Title Annotations Reprint: Utilization of Trifluorothymidine (TFT) to Select for Thymidine Kinase-Deficient Mutants from L5178Y/TK Mouse Lymphoma Cells.
PUB Date Free Form 1981
Category Codes 6T; 6C; 57Y; 57F
NTIS Prices Not available NTIS
Primary Description 600/11
Document Type NT
Control Number 417425593
Cataloging Source NTIS/MT
Origin NTIS
Type CAT