OLS : Record

RECORD NUMBER: 17 OF 20

Main Title Quantitation of Small Colony Trifluorothymidine-Resistant Mutants of L5178Y/TK+/- Mouse Lymphoma Cells in RPMI-1640 Medium.
Author Moore, M. M.; Howard, B. E.;
CORP Author Health Effects Research Lab., Research Triangle Park, NC.
Year Published 1982
Report Number EPA/600/J-82-115;
Stock Number PB84-101252
Subjects Lymphomas; Mutations; Mice; Cells(Biology); Reprints; Thymidine/trifluoro
Holdings
Library   Call Number Additional Info Location Date Modified
NTIS PB84-101252 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 06/23/1988
Collation 10p
Abstract The L5178Y/TK t/-mouse lymphoma assay quantitates the induction of thymidine kinase deficient (TK-/-) mutants. When plated in Fischer's medium for leukemic cells of mice, mutants appear morphologically as large and small trifluorothymidine-resistant (TFT) colonies; mutants iolated in Fischer's medium using 1 ug/ml TFT are TK deficient and TFT-resistant upon rechallenge with TFT. Cytogenetic analysis has revealed that mutants represent chromosomal mutants of chromosome 11, whereas mutants appear to represent single gene mutations. A number of laboratories have preferred to use RPMI-1640 medium for the L5178Y/TKt/-assay instead of Fischer's medium.
Supplementary Notes Pub. in Mutation Research, 104 p287-294 1982.
NTIS Title Notes Journal article.
Title Annotations Reprint: Quantitation of Small Colony Trifluorothymidine-Resistant Mutants of L5178Y/TK+/- Mouse Lymphoma Cells in RPMI-1640 Medium.
PUB Date Free Form 1982
Category Codes 6C; 57F
NTIS Prices PC A02/MF A01
Primary Description 600/11
Document Type NT
Control Number 400621118
Cataloging Source NTIS/MT
Origin NTIS
Type CAT