| Main Title |
Quantitation of Small Colony Trifluorothymidine-Resistant Mutants of L5178Y/TK+/- Mouse Lymphoma Cells in RPMI-1640 Medium. |
| Author |
Moore, M. M.;
Howard, B. E.;
|
| CORP Author |
Health Effects Research Lab., Research Triangle Park, NC. |
| Year Published |
1982 |
| Report Number |
EPA/600/J-82-115; |
| Stock Number |
PB84-101252 |
| Subjects |
Lymphomas;
Mutations;
Mice;
Cells(Biology);
Reprints;
Thymidine/trifluoro
|
| Holdings |
| Library |
|
Call Number |
Additional Info |
Location |
Date Modified |
| NTIS |
|
PB84-101252 |
Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. |
NTIS |
06/23/1988 |
|
| Collation |
10p |
| Abstract |
The L5178Y/TK t/-mouse lymphoma assay quantitates the induction of thymidine kinase deficient (TK-/-) mutants. When plated in Fischer's medium for leukemic cells of mice, mutants appear morphologically as large and small trifluorothymidine-resistant (TFT) colonies; mutants iolated in Fischer's medium using 1 ug/ml TFT are TK deficient and TFT-resistant upon rechallenge with TFT. Cytogenetic analysis has revealed that mutants represent chromosomal mutants of chromosome 11, whereas mutants appear to represent single gene mutations. A number of laboratories have preferred to use RPMI-1640 medium for the L5178Y/TKt/-assay instead of Fischer's medium. |
| Supplementary Notes |
Pub. in Mutation Research, 104 p287-294 1982. |
| NTIS Title Notes |
Journal article. |
| Title Annotations |
Reprint: Quantitation of Small Colony Trifluorothymidine-Resistant Mutants of L5178Y/TK+/- Mouse Lymphoma Cells in RPMI-1640 Medium. |
| PUB Date Free Form |
1982 |
| Category Codes |
6C; 57F |
| NTIS Prices |
PC A02/MF A01 |
| Primary Description |
600/11 |
| Document Type |
NT |
| Control Number |
400621118 |
| Cataloging Source |
NTIS/MT |
| Origin |
NTIS |
| Type |
CAT |
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