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OLS : Record

RECORD NUMBER: 11 OF 19

Main Title Isolation of the 'Candida tropicalis' Gene for P450 Lanosterol Demethylase and Its Expression in 'Saccharomyces cerevisiae'.
Author Chen, C.; Turi, T. G.; Sanglard, D.; Loper, J. C.;
CORP Author Cincinnati Univ. Medical Center, OH. Dept. of Microbiology and Molecular Genetics.;Environmental Protection Agency, Cincinnati, OH. Risk Reduction Engineering Lab.
Publisher c1987
Year Published 1987
Report Number EPA-CR-810605 ;EPA-CR-813366; EPA/600/J-93/303;
Stock Number PB93-229250
Subjects Cytochrome P-450; Saccharomyces cerevisiae; Fungal genes; Gene expression; Escherichia coli; Fungal DNA; Heme; Drug resistance; Ketoconazole; Molecular sequence data; Reprints; Candida tropicalis
Holdings
Library   Call Number Additional Info Location Date Modified
NTIS PB93-229250 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 11/22/1993
Collation 9p
Abstract The gene for cytochrome P450 lanosterol 14alpha-demethylase (14DM) from the yeast Candida tropicalis has been isolated. This was accomplished by screening genomic libraries of strain ATCC750 in E. coli using a DNA fragment containing the yeast Saccharomyces cerevisiae 14DM gene. Identity of this gene was confirmed by (a) observing a heme binding region common to all P450s after sequencing the 3' portion of the gene, and (b) based upon tests of its expression in strains of Saccharomyces cerevisiae. (Copyright (c) 1987 Academic Press, Inc.)
Supplementary Notes Pub. in Biochemical and Biophysical Research Communications, v146 n3 p1311-1317 Aug 87. See also PB93-229284. Sponsored by Environmental Protection Agency, Cincinnati, OH. Risk Reduction Engineering Lab.
NTIS Title Notes Journal article.
Title Annotations Reprint: Isolation of the 'Candida tropicalis' Gene for P450 Lanosterol Demethylase and Its Expression in 'Saccharomyces cerevisiae'.
Category Codes 57F; 57K
NTIS Prices PC A02/MF A01
Primary Description 600/14
Document Type NT
Control Number 328023339
Cataloging Source NTIS/MT
Origin NTIS
Type CAT


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