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RECORD NUMBER: 4 OF 6

Main Title Transfection of a Rat Cytochrome P450b cDNA into C3H10T1/2CL8 Mouse Embryo Fibroblasts.
Author Hansen, S. K.; Ross, J. A.; Siegried, J. M.; Leavitt, S.; Rudo, K.;
CORP Author Environmental Health Research and Testing, Inc., Research Triangle Park, NC. ;National Inst. of Environmental Health Sciences, Research Triangle Park, NC.;Health Effects Research Lab., Research Triangle Park, NC.
Publisher c1989
Year Published 1989
Report Number EPA-68-02-4456; EPA/600/J-89/358;
Stock Number PB90-216060
Subjects Embryo; Rats; Mice; Fibroblasts; Carcinogens; Metabolism; Reprints; Transfection; Cytochrome P-450; cDNA; 2-acetylaminofluorene; Clone cells; Promotor regions(Genetics); Plasmids; Cell survival; Southern immunoblotting; Northern immunoblotting
Holdings
Library   Call Number Additional Info Location Date Modified
NTIS PB90-216060 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 08/27/1990
Collation 9p
Abstract A cDNA clone of a rat cytochrome P450b gene was used to construct an expression vector driven by an SV40 promoter and containing a G418-resistance selectable marker. This bifunctional plasmid (pJRSL100) was transfected into the C3H10T1/2CL8 mouse embryo fibroblast cell line. G418-resistant clones were selected and tested for enhanced sensitivity to the cytotoxic effects of the carcinogens 2-acetylaminofluorene (2-AAF) and N,N-dimethylnitrosamine (DMN), compounds which normally do not induce cytotoxicity nor morphological transformation in these cells. DNA analyses of one subclone, 19P450b-4, which exhibited increased cytotoxic responses to 2-AAF and DMN compared to the parental C3H10T1/2CL8 cells, demonstrated an increase in the number of copies of the cytochrome P450b and demonstrated the appearance of unique restriction fragment bands relative to parental and control transfected cells. This subclone also exhibited increased levels of mRNA complementary to the P450b cDNA. (Copyright (c) 1989 Alan R. Liss, Inc.)
Supplementary Notes Pub. in Jnl. of Molecular Carcinogenesis, v2 p261-267 Nov 89. Prepared in cooperation with National Inst. of Environmental Health Sciences, Research Triangle Park, NC. Sponsored by Health Effects Research Lab., Research Triangle Park, NC.
NTIS Title Notes Journal article.
Title Annotations Reprint: Transfection of a Rat Cytochrome P450b cDNA into C3H10T1/2CL8 Mouse Embryo Fibroblasts.
Category Codes 57F; 57Y
NTIS Prices PC A02/MF A01
Primary Description 600/10
Document Type NT
Control Number 019410512
Cataloging Source NTIS/MT
Origin NTIS
Type CAT


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