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Main Title Potential of Carp to Bioactivate Benzo(a)pyrene to Metabolites That Bind to DNA.
Author Kurelec, B.; Krca, S.; Garg, A.; Gupta, R. C.;
CORP Author Institut Rudjer Boskovic, Zagreb (Yugoslavia). Center for Marine Research. ;Kentucky Univ., Lexington. Graduate Center for Toxicology.;Health Effects Research Lab., Research Triangle Park, NC.
Publisher c1991
Year Published 1991
Report Number EPA-R-816185; EPA/600/J-92/197;
Stock Number PB92-189034
Subjects Benzo(a)pyrene; Carp; Metabolic activation; Deoxyribonucleic acids; Benzoflavones; Mixed function oxidases; Liquid chromatography; Phosphorus 32; Aromatic polycylic hydrocarbons; Toxic substances; Reprints;
Holdings
Library   Call Number Additional Info Location Date Modified
NTIS PB92-189034 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 08/22/1992
Collation 8p
Abstract The authors have investigated the formation of DNA adducts in starved, fed, and 5,6-benzoflavone-pretreated carp following i.p. administration of benzo(a)pyrene. (32)P-postlabeling analysis of the liver DNAs showed the presence of one predominant (>92%) adduct in all three groups. Cochromatography experiments revealed that the main adduct was identical to authentic BPDEI-dG (10(beta)-(deoxyguanosin-N2-yl)-7(beta), 8(alpha), 9(alpha)-trihydroxy-7,8,0,10-tetrahydrobenzo(a)pyrene). The formation of the adduct was evident as early as 1.5 h post-treatment and the levels increased steadily up to 7 days, reaching about 125, 110, and 102 attomole/microgram DNA in starved, fed, and benzoflavone-pretreated carp, respectively. During this period, the benzo(a)pyrene-induced benzo(a)pyrene monooxygenase activity increased from the uninduced, natural level of about 3 pmol/mg per min to levels of 35, 62, and 79 pmol/mg per min in starving, fed, and 5,6-benzoflavone pretreated fish, respectively. A slow but steady formation of the diolepoxide-dG adduct was also observed in the liver DNA of carp following p.o. treatment. (Copyright (c) 1991 Elsevier Scientific Publishers Ireland Ltd.)
Supplementary Notes Pub. in Jnl. Cancer Letters, v57 p255-260 1991. Prepared in cooperation with Kentucky Univ., Lexington. Graduate Center for Toxicology. Sponsored by Health Effects Research Lab., Research Triangle Park, NC.
NTIS Title Notes Journal article.
Title Annotations Reprint: Potential of Carp to Bioactivate Benzo(a)pyrene to Metabolites That Bind to DNA.
Category Codes 57B; 57F; 57Y
NTIS Prices PC A02/MF A01
Primary Description 600/10
Document Type NT
Control Number 220521585
Cataloging Source NTIS/MT
Origin NTIS
Type CAT


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