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Main Title

Potential of Carp to Bioactivate Benzo(a)pyrene to Metabolites That Bind to DNA.

Author

Kurelec, B.; Krca, S.; Garg, A.; Gupta, R. C.;

CORP Author

Institut Rudjer Boskovic, Zagreb (Yugoslavia). Center for Marine Research. ;Kentucky Univ., Lexington. Graduate Center for Toxicology.;Health Effects Research Lab., Research Triangle Park, NC.

Publisher

c1991

Year Published

1991

Report Number

EPA-R-816185; EPA/600/J-92/197;

Stock Number

PB92-189034

Subjects

Benzo(a)pyrene; Carp; Metabolic activation; Deoxyribonucleic acids; Benzoflavones; Mixed function oxidases; Liquid chromatography; Phosphorus 32; Aromatic polycylic hydrocarbons; Toxic substances; Reprints;

Holdings

Library		Call Number	Additional Info	Location	Date Modified
NTIS		PB92-189034	Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy.	NTIS	08/22/1992

Collation

Abstract

The authors have investigated the formation of DNA adducts in starved, fed, and 5,6-benzoflavone-pretreated carp following i.p. administration of benzo(a)pyrene. (32)P-postlabeling analysis of the liver DNAs showed the presence of one predominant (>92%) adduct in all three groups. Cochromatography experiments revealed that the main adduct was identical to authentic BPDEI-dG (10(beta)-(deoxyguanosin-N2-yl)-7(beta), 8(alpha), 9(alpha)-trihydroxy-7,8,0,10-tetrahydrobenzo(a)pyrene). The formation of the adduct was evident as early as 1.5 h post-treatment and the levels increased steadily up to 7 days, reaching about 125, 110, and 102 attomole/microgram DNA in starved, fed, and benzoflavone-pretreated carp, respectively. During this period, the benzo(a)pyrene-induced benzo(a)pyrene monooxygenase activity increased from the uninduced, natural level of about 3 pmol/mg per min to levels of 35, 62, and 79 pmol/mg per min in starving, fed, and 5,6-benzoflavone pretreated fish, respectively. A slow but steady formation of the diolepoxide-dG adduct was also observed in the liver DNA of carp following p.o. treatment. (Copyright (c) 1991 Elsevier Scientific Publishers Ireland Ltd.)

Supplementary Notes

Pub. in Jnl. Cancer Letters, v57 p255-260 1991. Prepared in cooperation with Kentucky Univ., Lexington. Graduate Center for Toxicology. Sponsored by Health Effects Research Lab., Research Triangle Park, NC.

NTIS Title Notes

Journal article.

Title Annotations

Reprint: Potential of Carp to Bioactivate Benzo(a)pyrene to Metabolites That Bind to DNA.

Category Codes

57B; 57F; 57Y

NTIS Prices

PC A02/MF A01

Primary Description

600/10

Document Type

Control Number

220521585

Cataloging Source

NTIS/MT

Origin

NTIS

Type

CAT

OLS : Record

RECORD NUMBER: 1 OF 3

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