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Main Title Environmental technology verification report: PCB detection technology {microform} : Hybrizyme Delfia (trademart) PCB assay /
Author Dindal, Amy B.; Bayne, C. K.; Jenkins, R. A.; Koglin, E. N.
CORP Author Oak Ridge National Lab., TN.;Environmental Protection Agency, Las Vegas, NV. National Exposure Research Lab.
Publisher U.S. Environmental Protection Agency, Office of Research and Development, National Exposure Research Laboratory, Environmental Sciences Division,
Place Published Las Vegas, NV. :
Year Published 2000
Report Number EPA/600/R-01/052; NERL/LV-01-086
Stock Number PB2002-100442
OCLC Number 48887982
Subjects Polychlorinated biphenyls; Technology verification; Analytical techniques; Solvent extracts; Logistical operations; Monitoring; Verification tests; Laboratory analyses; Immunoassay kits; Environmental Protection Agency; DELFIA PCB Assay; Fluoroimmunoassay; Monoclonal antibody reagents
Holdings
Library   Call Number Additional Info Location Date Modified
ERBD PB2002-100442 NERL/ESD-LV Library/Las Vegas,NV 02/01/2002
NTIS PB2002-100442 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 01/01/1988
Collation 50 p. ; ills. : 28 cm.
Abstract The DELFIA PCB Assay is a solid-phase time-resolved fluoroimmunoassay based on the sequential addition of sample extract and europium-labeled PCB tracer to a monoclonal antibody reagent specific for PCBS. In this assay, the antibody reagent and sample extract are added to a strip of Microtiter plate wells and allowed to react. The strips have been specially treated to trap the antibody reagent or antibody-PCB complexes that may have formed. A wash step removes sample matrix from the captured antibody. This step significantly reduces any potential matrix interferences before the addition of the PCB tracer, resulting in an unusually robust assay system. The PCB tracer is then added and allowed to bind the antibodies that are not complexed with sample PCBS. A wash step is used to separate antibody-bound tracer from the tracer free in solution. The addition of an enhancement solution forms highly fluorescent chelates with the bound europium ions. The amount of fluorescence measured is inversely proportional to the concentration of PCBs in the sample. The lower reporting level is typically 0.5 ppm.
Notes Cover title. "August 2001." "EPA/600/R-01/052." Microfiche.
Supplementary Notes Sponsored by Environmental Protection Agency, Las Vegas, NV. National Exposure Research Lab.
Availability Notes Product reproduced from digital image. Order this product from NTIS by: phone at 1-800-553-NTIS (U.S. customers); (703)605-6000 (other countries); fax at (703)605-6900; and email at orders@ntis.gov. NTIS is located at 5285 Port Royal Road, Springfield, VA, 22161, USA.
Author Added Ent
Bayne, Charles K.;
Jenkins, Roger A.;
Koglin, Eric N.
Corporate Au Added Ent National Exposure Research Laboratory Environmental Sciences Division, Las Vegas, Nev. ; United States. Environmental Protection Agency. Office of Research and Development. ; Oak Ridge National Laboratory.
Alternate Title PCB detection technology. Hybrizyme delfia PCB assay.
PUB Date Free Form 2001.
NTIS Prices PC A04/MF A01
BIB Level m
OCLC Time Stamp 20020129132925
Cataloging Source OCLC/T
Language eng
Origin OCLC
Type MERGE
OCLC Rec Leader 01499nam 2200337Ka 45020