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Main Title Mammalian Cell Gene Mutation Assays Working Group Report.
Author Aaron, C. S.; Bolcsfoldi, G.; Glatt, H. R.; Moore, M.; Nishi, Y.;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. ;Upjohn Co., Kalamazoo, MI. ;Mainz Univ. (Germany, F.R.). ;Pharmakon Research International, Inc., Waverly, PA. ;Warner-Lambert Co., Ann Arbor, MI. Parke-Davis Pharmaceutical Research Div.
Publisher c1994
Year Published 1994
Report Number EPA/600/J-94/418;
Stock Number PB95-128047
Subjects Mutation; Mammals; Cells(Biology); Mutagenesis; Mutagenicity tests; Metabolic activation; Lymphoma; Thymidine kinase; Reproducibility tests; Reprints;
Holdings
Library   Call Number Additional Info Location Date Modified
NTIS PB95-128047 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 03/06/1995
Collation 10p
Abstract Mammalian cell gene mutation assays have been used for many years and the diversity of the available systems attests to the varied methods found to grow mammalian cells and detect mutations. As part of the International Workshop on Standardization of Genotoxicity Test Procedures, in Melbourne, Australia, 27-28 February, 1993, various international guidelines were examined with respect to protocol issues. Mammalian cell gene mutation assays generally detect cells which are able to survive in the presence of a selective agent only when a newly arising mutation occurs. Five topic areas were discussed: (4) dose setting, (2) test systems of choice, (3) study design, (4) repetition of tests and (5) criteria of final results. In general, the group felt that the protocols for the conduct of specific gene mutation assays in mammalian cells have been worked out independently and that many aspects of protocol design are not highly contested. Consensus was reached in many areas with respect to elements of protocol design for assays for mutation induction using mammalian cells in culture. A number of issues of a generic nature, common to all in vitro assays was discussed by the larger group.
Supplementary Notes Pub. in Mutation Research 312, n3 p235-239 Jun 94. Prepared in cooperation with Upjohn Co., Kalamazoo, MI., Mainz Univ. (Germany, F.R.)., Pharmakon Research International, Inc., Waverly, PA., and Warner-Lambert Co., Ann Arbor, MI. Parke-Davis Pharmaceutical Research Div.
Highlights Notes %AUT:L. /Stankowski ;J. /Theiss ;E. /Thompson
Title Annotations Reprint: Mammalian Cell Gene Mutation Assays Working Group Report.
Category Codes 57F
NTIS Prices PC A02/MF A01
Primary Description 600/10
Document Type NT
Control Number 500618272
Cataloging Source NTIS/MT
Origin NTIS
Type CAT