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Main Title Developmental Toxicity of Methanol in Whole Embryo Culture: A Comparative Study with Mouse and Rat Embryos.
Author Andrews, J. E.; Ebron-McCoy, M.; Logsdon, T. R.; Mole, L. M.; Kavlock, R. J.;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. Developmental Toxicology Div. ;ManTech Environmental Technology, Inc., Research Triangle Park, NC.
Publisher 1993
Year Published 1993
Report Number EPA/600/J-94/029;
Stock Number PB94-137254
Subjects Methanol; Teratogens; Embryos; Comparison; Rats; Mice; Species diversity; Fetal development; Reprints;
Holdings
Library   Call Number Additional Info Location Date Modified
NTIS PB94-137254 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 05/14/1994
Collation 12p
Abstract Methanol (MeOH), a widely used industrial solvent, has been proposed as an alternative motor vehicle fuel. Inhaled MeOH is developmentally toxic in both rats and nice but the mouse is more sensitive than is the rat. The contribution of the embryo to this differential sensitivity was studied in whole embryo culture (WEC) using equivalent stage rat (day 9) and mouse (day 8) embryos (plug day = day 0). Rat embryos were explanted and cultured in 0, 2, 4, 8, 12 or 16 mg MeOH/ml rat serum for 24 h and then transferred to rat serum alone for 24 h. Embryonic development of the 2 and 4 mg MeOH/ml groups was not significantly different from the controls whereas the higher concentrations resulted in a concentration related decrease in somite number, head length and developmental score. The 12 mg/ml dose resulted in some embryolethality as well as dysmorphogenesis, while the highest dose was embryolethal. MeOH was dysmorphogenic in vitro in rat embryos at a MeOH concentration comparable to that reported in maternal serum following teratogenic in vivo exposures. Day 8 mouse embryos were explanted and cultured in 0, 2, 4, 6 or 8 mg MeOH/ml culture medium (75% rat serum, 25% Tyrode's salt solution) for 24 h. Embryonic development in the 2 mg/ml MeOH group was not significantly different from the controls but all higher concentration groups had a significant decrease in developmental score and crown-rump length. The high concentration group also suffered 80% embryolethality. (Copyright (c) 1993 Elsevier Scientific Publishers Ireland Ltd.)
Supplementary Notes Pub. in Toxicology, v81 n3 p205-215, 27 Aug 93. Prepared in cooperation with ManTech Environmental Technology, Inc., Research Triangle Park, NC.
NTIS Title Notes Journal article.
Title Annotations Reprint: Developmental Toxicity of Methanol in Whole Embryo Culture: A Comparative Study with Mouse and Rat Embryos.
Category Codes 57Y; 57S
NTIS Prices PC A03/MF A01
Primary Description 600/10
Document Type NT
Control Number 407720042
Cataloging Source NTIS/MT
Origin NTIS
Type CAT


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