OLS : Record

RECORD NUMBER: 3 OF 3

Main Title

Transfer of Arsenite from Glutathione to Dithiols: A Model of Interaction.

Author

Delnomdedieu, M.; Basti, M. M.; Otvos, J. D.; Thomas, D. J.;

CORP Author

North Carolina Univ. at Chapel Hill. Center for Environmental Medicine and Lung Biology. ;North Carolina State Univ. at Raleigh. Dept. of Biochemistry.;Health Effects Research Lab., Research Triangle Park, NC. Pharmacokinetics Branch.

Publisher

cOct 93

Year Published

1993

Report Number

EPA-R-817643; EPA/600/J-94/532;

Stock Number

PB95-148946

Subjects

Glutathione; Arsenites; Chemical models; Thiols; Nuclear magnetic resonance spectroscopy; Binding sites; Arsenates; Reduction(Chemistry); Carbon 13; Reprints; Succinic acid/meso-2,3-dimercapto

Holdings

Library		Call Number	Additional Info	Location	Date Modified
NTIS		PB95-148946	Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy.	NTIS	03/06/1995

Collation

Abstract

The interactions of arsenate and arsenite with meso-2,3-dimercaptosuccinic acid (DMSA) have been characterized using carbon-13 nuclear magnetic resonance. These studies show that DMSA reduces arsenate to arsenite and complexes arsenite. Monitoring the carbon-13 signals of complexed DMSA and liberated glutathione shows that DMSA readily extracts arsenite from a glutathione(sub 3)-arsenite complex, proving the affinity of arsenite for dithiols is greater than that for monothiols. Competition between DMSA (vicinal thiols) and dithioerythritol (1,4-dimercapto-2,3-butanediol) for binding of arsenite indicates that the binding affinity is inversely related to the distance between the two thiol groups. On the basis of these findings, a model for the interaction of arsenic with mono- and dithiol-containing molecules is proposed. (Copyright (c) 1993 by the American Chemical Society.)

Supplementary Notes

Pub. in Chemical Research in Toxicology 6, n5 p598-602 Oct 93. Prepared in cooperation with North Carolina State Univ. at Raleigh. Dept. of Biochemistry. Sponsored by Health Effects Research Lab., Research Triangle Park, NC. Pharmacokinetics Branch.

NTIS Title Notes

Journal article.

Title Annotations

Reprint: Transfer of Arsenite from Glutathione to Dithiols: A Model of Interaction.

Category Codes

99D

NTIS Prices

PC A02/MF A01

Primary Description

600/10

Document Type

Control Number

503415431

Cataloging Source

NTIS/MT

Origin

NTIS

Type

CAT

OLS : Record

RECORD NUMBER: 3 OF 3

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