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RECORD NUMBER: 3 OF 3

Main Title Transfer of Arsenite from Glutathione to Dithiols: A Model of Interaction.
Author Delnomdedieu, M.; Basti, M. M.; Otvos, J. D.; Thomas, D. J.;
CORP Author North Carolina Univ. at Chapel Hill. Center for Environmental Medicine and Lung Biology. ;North Carolina State Univ. at Raleigh. Dept. of Biochemistry.;Health Effects Research Lab., Research Triangle Park, NC. Pharmacokinetics Branch.
Publisher cOct 93
Year Published 1993
Report Number EPA-R-817643; EPA/600/J-94/532;
Stock Number PB95-148946
Subjects Glutathione; Arsenites; Chemical models; Thiols; Nuclear magnetic resonance spectroscopy; Binding sites; Arsenates; Reduction(Chemistry); Carbon 13; Reprints; Succinic acid/meso-2,3-dimercapto
Holdings
Library   Call Number Additional Info Location Date Modified
NTIS PB95-148946 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 03/06/1995
Collation 6p
Abstract The interactions of arsenate and arsenite with meso-2,3-dimercaptosuccinic acid (DMSA) have been characterized using carbon-13 nuclear magnetic resonance. These studies show that DMSA reduces arsenate to arsenite and complexes arsenite. Monitoring the carbon-13 signals of complexed DMSA and liberated glutathione shows that DMSA readily extracts arsenite from a glutathione(sub 3)-arsenite complex, proving the affinity of arsenite for dithiols is greater than that for monothiols. Competition between DMSA (vicinal thiols) and dithioerythritol (1,4-dimercapto-2,3-butanediol) for binding of arsenite indicates that the binding affinity is inversely related to the distance between the two thiol groups. On the basis of these findings, a model for the interaction of arsenic with mono- and dithiol-containing molecules is proposed. (Copyright (c) 1993 by the American Chemical Society.)
Supplementary Notes Pub. in Chemical Research in Toxicology 6, n5 p598-602 Oct 93. Prepared in cooperation with North Carolina State Univ. at Raleigh. Dept. of Biochemistry. Sponsored by Health Effects Research Lab., Research Triangle Park, NC. Pharmacokinetics Branch.
NTIS Title Notes Journal article.
Title Annotations Reprint: Transfer of Arsenite from Glutathione to Dithiols: A Model of Interaction.
Category Codes 99D
NTIS Prices PC A02/MF A01
Primary Description 600/10
Document Type NT
Control Number 503415431
Cataloging Source NTIS/MT
Origin NTIS
Type CAT