||Development of In vivo Genotoxicity Tests in Estuarine Fish and Their Application to Aquatic Toxicology.
Means, J. C.;
Daniels, C. B.;
Baksi, S. M.;
||Louisiana State Univ., Baton Rouge. Inst. for Environmental Studies. ;Johns Hopkins Univ., Baltimore, MD. School of Hygiene and Public Health.;Environmental Research Lab., Narragansett, RI.
||EPA/600/J-88/463 ; ERLN-P06
In vivo analysis;
||Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy.
||Genotoxicity assays were developed in embryonic stages of two species of fish, striped bass (Morone saxitilis) and sheepshead minnow (Cyprinodon variegatus), and in the adults of Fundulus heteroclitus using metaphase chromosome aberrations as the endpoint. Dose-dependent responses were obtained with several chemical mutagens, including 9-aminoacridine, ethylmethane sulphonate, cyclophosphamide and n-methyl-n-nitro-n-nitroguanidine, added to estuarine water at doses spanning several orders of magnitude in compound concentration. Exposures ranged from one to four days; however, two-day exposure time was found to be optimal in eggs and larvae. Tissues from the gills, kidney and intestinal tract of adult Fundulus were found to be responsive to mutagen exposure; however, the intestine gave the best responses. The results of these experiments suggest that these assays are sufficiently sensitive to be used in the field as well as in laboratory tests. (Copyright (c) 1988 Elsevier Applied Science Publishers, Ltd.)
||Pub. in Marine Environmental Research, v24 p327-331 1988. Prepared in cooperation with Johns Hopkins Univ., Baltimore, MD. School of Hygiene and Public Health. Sponsored by Environmental Research Lab., Narragansett, RI.
|NTIS Title Notes
||Reprint: Development of In vivo Genotoxicity Tests in Estuarine Fish and Their Application to Aquatic Toxicology.
||57Y; 57F; 68D; 47D
||PC A02/MF A01