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Main Title Simple Mini-Method to Extract DNA Directly from Soil for Use with Polymerase Chain Reaction Amplification.
Author Porteous, L. A.; Armstrong, J. L.;
CORP Author Corvallis Environmental Research Lab., OR.
Publisher c1993
Year Published 1993
Report Number EPA/600/J-93/288;
Stock Number PB93-222792
Subjects Deoxyribonucleic acids; Enzymes; Soils; Electrophoresis; Bacteria; Fungi; Extraction; Separation; Reprints; Polymerase chain reactions; Ecological studies
Holdings
Library   Call Number Additional Info Location Date Modified
NTIS PB93-222792 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 11/22/1993
Collation 6p
Abstract A rapid, simple method is used that yields amplifiable fungal and bacterial DNAs directly from soil. DNA is separated from soil contaminants by electrophoresis in low-melting-temperature agarose and used directly in polymerase chain reaction amplification. Fifty 20-mg samples can be processed in one day. Fragments of 16S and 18S ribosomal RNAs are amplified by polymerase chain reaction with DNA extracted from the soil. Universal primers are used that are capable of amplifying ribosomal DNA from a wide variety of bacteria and fungi. Eubacterial and fungal primers are used that are capable of distinguishing between eubacterial and fungal DNAs. Restriction enzyme digests are performed on amplified DNA fragments from five soil samples. (Copyright (c) Springer-Verlag Inc. 1993.)
Supplementary Notes Pub. in Current Microbiology, v27 p115-118 1993. See also PB92-108141.
NTIS Title Notes Journal article.
Title Annotations Reprint: Simple Mini-Method to Extract DNA Directly from Soil for Use with Polymerase Chain Reaction Amplification.
Category Codes 57K; 57H
NTIS Prices PC A02/MF A01
Primary Description 600/02
Document Type NT
Control Number 328020029
Cataloging Source NTIS/MT
Origin NTIS
Type CAT