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Main Title Environmental technology verification report: TNT detection technology {microform} : Texas Instruments Spreeta Sensor /
Author Dindal, Amy B.; Banyne, C. K.; Jenkins, R. A.; Koglin, E. N.
CORP Author Oak Ridge National Lab., TN.;Environmental Protection Agency, Las Vegas, NV. National Exposure Research Lab.
Publisher U.S. Environmental Protection Agency, Office of Research and Development, National Exposure Research Laboratory, Environmental Sciences Division,
Place Published Las Vegas, NV. :
Year Published 2001
Report Number EPA/600/R-01/064; NERL-LV-01-099
Stock Number PB2002-100441
OCLC Number 48887962
Subjects TNT; Detection; Verification; Soil remediation; Soil pollution; Characteristics; Monitoring; Chemical explosives; Nuclear magnetic resonance; Surfaces; Laboratory tests; Military facilities; Analytical chemistry; Soil sampling; Sensors; Calibration; Molecules
Holdings
Library   Call Number Additional Info Location Date Modified
ERBD PB2002-100441 NERL/ESD-LV Library/Las Vegas,NV 02/01/2002
NTIS PB2002-100441 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 01/01/1988
Collation various pagings ; ills. : 28 cm.
Abstract Spreeta is an integrated, miniaturized sensor platform which employs surface plasmon resonance (SPR) to detect changes in refractive index within a few thousand angstroms of the active gold surface. Specificty is provided by placing a thin biofihn on the sensor surface. For example, by placing an antibody to fluorescein on the sensor surface, the binding of fluorosceinated proteins, seen as a local increase in refractive index, is simply performed. SPR has been used in this way to study biomolecular binding events for more than a decade, but Spreeta is the first miniaturized SPR platform. TNT detection is most efficiently performed by methods other than direct binding. This is because on a molecule-for-molecule basis, small molecules are much less effective than large molecules at changing refractive index; thus, any direct SPR assay can detect large molecules at a lower concentration than it can detect small molecules. For this reason, Texas Instruments has developed a robust inhibition assay in which the presence of two TNT molecules (228 daltons) effectively inhibits the binding of one antibody molecule (I 50,000 daltons). To analyze a sample, 0.5g of soil is extracted in an aqueous solution. The assay starts with a conjugate of trinitrobenzene (TNB) and bovine serum albumin on the gold sensing surface. Assays are then performed by exposing that sensing surface to an anti-TNT antibody solution which may or may not contain free TNT. When free TNT is present, it binds to anti-TNT antibodies in solution and thereby keeps them from binding to the surface-bound TNT analog. This inhibited binding is compared to a reference run where the antibody solution did not contain free TNT. Results from this assay are reported as interval data (i.e., the concentration of TNT is between 0.3 and 0.9 rng/kg). The lowest reporting interval was 0 to 0.3 mg/kg.
Notes Cover title. "August 2001." "EPA/600/R-01/064." Microfiche.
Supplementary Notes Sponsored by Environmental Protection Agency, Las Vegas, NV. National Exposure Research Lab.
Availability Notes Product reproduced from digital image. Order this product from NTIS by: phone at 1-800-553-NTIS (U.S. customers); (703)605-6000 (other countries); fax at (703)605-6900; and email at orders@ntis.gov. NTIS is located at 5285 Port Royal Road, Springfield, VA, 22161, USA.
Author Added Ent
Bayne, Charles K.;
Jenkins, Roger A.;
Koglin, Eric N.
Corporate Au Added Ent National Exposure Research Laboratory Environmental Sciences Division, Las Vegas, Nev. ; United States. Environmental Protection Agency. Office of Research and Development. ; Oak Ridge National Laboratory.
Alternate Title TNT detection technology. Texas Instruments Spreeta Sensor.
PUB Date Free Form 2001.
NTIS Prices PC A04/MF A01
BIB Level m
OCLC Time Stamp 20020129132645
Cataloging Source OCLC/T
Language eng
Origin OCLC
Type MERGE
OCLC Rec Leader 01509nam 2200337Ka 45020