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RECORD NUMBER: 16 OF 8451

Main Title (32) P-Adduct Assay: Comparative Recoveries of Structurally Diverse DNA Adducts in the Various Enhancement Procedures.
Author Gupta, R. C.; Earley, K.;
CORP Author Baylor Coll. of Medicine, Houston, TX.;Health Effects Research Lab., Research Triangle Park, NC.
Publisher c1988
Year Published 1988
Report Number EPA/600/J-88/474;
Stock Number PB90-135385
Subjects Toxicology; Deoxyribonucleic acids; Carcinogens; Tables(Data); Thin layer chromatography; Reprints; DNA damage; Phosphorus radiosotopes; Autoradiography; Polynucleotide kinase
Holdings
Library   Call Number Additional Info Location Date Modified
NTIS PB90-135385 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 03/10/1990
Collation 9p
Abstract A (32)P-adduct assay for the measurement of low levels (I adduct per 10(7) nucleotides) of binding of carcinogens to DNA has been reported previously. The recovery pattern of most adducts examined in the polynucleotide kinase-enrichment assay was essentially the same as found in nuclease P1-mediated assay, except that overall lower values were obtained. The data suggest that a given DNA sample should be analyzed by different versions of the 32P-adduct assay, particularly DNA of specimens of humans exposed to low levels of unknown carcinogens. The observation that chemical structure of an adduct may be detrimental in its recovery in the enzyme- and extraction-mediated enrichment procedures may serve as a probe in the structural characterization of adducts of unknown carcinogens. (Copyright (c) Press Limited, Oxford, England.)
Supplementary Notes Pub. in Carcinogenesis, v9 n9 p1687-1693 1988. Sponsored by Health Effects Research Lab., Research Triangle Park, NC.
NTIS Title Notes Journal article.
Title Annotations Reprint: (32) P-Adduct Assay: Comparative Recoveries of Structurally Diverse DNA Adducts in the Various Enhancement Procedures.
Category Codes 57Y; 57F
NTIS Prices PC A02/MF A01
Primary Description 600/10
Document Type NT
Control Number 004019216
Cataloging Source NTIS/MT
Origin NTIS
Type CAT


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